A solubility optimization protocol for two-dimensional gel electrophoresis of cardiac tissue

Brian A. Stanley, Jennifer E. Van Eyk

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

We outline a strategy for the optimization of buffer conditions for the solubilization, extraction, and isoelectric focusing (IEF) of proteins from cardiac tissue for two-dimensional gel electrophoresis (2DE). This strategy, which involves altering both the extraction and IEF buffers, allows one to ensure representation of the proteome that is as complete as possible. Initial buffer choices are given, as well as basic protocols for modifications. Although these conditions have been effectively demonstrated for human myocardium, in principle this procedure can be used for the initial screen of any new sample of tissue or cultured cells.

Original languageEnglish (US)
Title of host publicationCardiovascular Proteomics
Subtitle of host publicationMethods and Protocols
EditorsFernando Vivanco
Pages59-65
Number of pages7
DOIs
StatePublished - Feb 5 2007

Publication series

NameMethods in Molecular Biology
Volume357
ISSN (Print)1064-3745

Keywords

  • 2DE
  • Heart muscle
  • IEF
  • Optimization
  • Solubilization

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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