A solubility optimization protocol for two-dimensional gel electrophoresis of cardiac tissue

Brian A. Stanley, Jennifer E. Van Eyk

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

We outline a strategy for the optimization of buffer conditions for the solubilization, extraction, and isoelectric focusing (IEF) of proteins from cardiac tissue for two-dimensional gel electrophoresis (2DE). This strategy, which involves altering both the extraction and IEF buffers, allows one to ensure representation of the proteome that is as complete as possible. Initial buffer choices are given, as well as basic protocols for modifications. Although these conditions have been effectively demonstrated for human myocardium, in principle this procedure can be used for the initial screen of any new sample of tissue or cultured cells.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
Pages59-65
Number of pages7
Volume357
DOIs
StatePublished - Feb 5 2007
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume357
ISSN (Print)10643745

Fingerprint

Electrophoresis, Gel, Two-Dimensional
Solubility
Buffers
Isoelectric Focusing
Proteome
Cultured Cells
Myocardium
Proteins

Keywords

  • 2DE
  • Heart muscle
  • IEF
  • Optimization
  • Solubilization

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Stanley, B. A., & Van Eyk, J. E. (2007). A solubility optimization protocol for two-dimensional gel electrophoresis of cardiac tissue. In Methods in Molecular Biology (Vol. 357, pp. 59-65). (Methods in Molecular Biology; Vol. 357). https://doi.org/10.1385/1-59745-214-9:59

A solubility optimization protocol for two-dimensional gel electrophoresis of cardiac tissue. / Stanley, Brian A.; Van Eyk, Jennifer E.

Methods in Molecular Biology. Vol. 357 2007. p. 59-65 (Methods in Molecular Biology; Vol. 357).

Research output: Chapter in Book/Report/Conference proceedingChapter

Stanley, BA & Van Eyk, JE 2007, A solubility optimization protocol for two-dimensional gel electrophoresis of cardiac tissue. in Methods in Molecular Biology. vol. 357, Methods in Molecular Biology, vol. 357, pp. 59-65. https://doi.org/10.1385/1-59745-214-9:59
Stanley BA, Van Eyk JE. A solubility optimization protocol for two-dimensional gel electrophoresis of cardiac tissue. In Methods in Molecular Biology. Vol. 357. 2007. p. 59-65. (Methods in Molecular Biology). https://doi.org/10.1385/1-59745-214-9:59
Stanley, Brian A. ; Van Eyk, Jennifer E. / A solubility optimization protocol for two-dimensional gel electrophoresis of cardiac tissue. Methods in Molecular Biology. Vol. 357 2007. pp. 59-65 (Methods in Molecular Biology).
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