A single subcutaneous inoculation of 107 armadillo-derived irradiated Mycobacterium leprae evokes different immunological behavior in C57Bl/6 and C3H mice

P. H. Lagrange, B. Hurtrel, P. Ravisse, J. Grosset

Research output: Contribution to journalArticle

Abstract

Following subcutaneous injection in the footpad with 6.7 x 103 living Mycobacterium leprae, it was observed that locally, at the injection site, more microorganisms were destroyed after the sixth month in C57BL/6 mice than in C3H/HeN mice. The specific and non-specific immune responses after inoculations of Armadillo-derived, irradiated killed M. leprae (IML) was then investigated in these two strains of mice and also in Biozzi's HL and LL mice. One single injection of 1 x 107 IML into the hind footpad of C57BL/6 and Biozzi's HL mice was able to elicit a state of cell-mediated immunity (CMI) to M. leprae antigens or to cross-reacting mycobacterial antigens, such as BCG antigens. The selected parameters of CMI were as follows. Development of an immune granuloma at the injection site and in the draining lymph node which corresponds to the accumulation of macrophages and lymphoid cells. Development of a systemic delayed-type hypersensitivity (DTH) to both living BCG and IML. Specific acquired protection against infection with M. bovis strain BCG. Immunopotentiation of the DTH response to an unrelated antigen, such as sheep red blood cells. In low responder strains (C3H/HeN and low-antibody producer mice selected by Biozzi) no such parameters developed at all after immunization. The mechanisms and relevance of these observations to the efficacy of an antileprosy vaccine for man is discussed.

Original languageEnglish (US)
Pages (from-to)167-168
Number of pages2
JournalAnnales de Microbiologie
Volume133 B
Issue number1
StatePublished - 1982
Externally publishedYes

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Armadillos
Mycobacterium leprae
Inbred C3H Mouse
Biozzi Mice
Mycobacterium bovis
Antigens
Delayed Hypersensitivity
Cellular Immunity
Injections
Subcutaneous Injections
Granuloma
Inbred C57BL Mouse
Immunization
Sheep
Erythrocytes
Lymph Nodes
Macrophages
Lymphocytes
Antibodies
Infection

ASJC Scopus subject areas

  • Medicine(all)

Cite this

A single subcutaneous inoculation of 107 armadillo-derived irradiated Mycobacterium leprae evokes different immunological behavior in C57Bl/6 and C3H mice. / Lagrange, P. H.; Hurtrel, B.; Ravisse, P.; Grosset, J.

In: Annales de Microbiologie, Vol. 133 B, No. 1, 1982, p. 167-168.

Research output: Contribution to journalArticle

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