A Single-Scan Inhomogeneity-Tolerant NMR Method for High-Resolution Two-Dimensional J-Resolved Spectroscopy

Haolin Zhan, Xiaoqing Lin, Zhiliang Wei, Qimiao Ye, Shuhui Cai, Xueqiu You, Yuqing Huang, Zhong Chen

Research output: Contribution to journalArticlepeer-review


Objective: A robust and general single-scan NMR method, SGEN-J, is proposed for real-time recording high-resolution two-dimensional (2D) homonuclear J-resolved spectra under inhomogeneous magnetic fields. Methods: This proposed NMR method is designed based on the combination of a selective gradient encoding module to encode chemical shifts with spatial positions, and a J-modulation decoding module to reveal encoded structural information. Multi-band SGEN-J is further implemented to effectively enhance spectral sensitivity with a sustained tolerance of field inhomogeneity. Results: The SGEN-J provides an effective way to rapidly recover chemical shifts, J coupling constants, and multiplet patterns under inhomogeneous magnetic fields. Experiments on various chemical solutions were performed to demonstrate the feasibility and effectiveness of SGEN-J. Experiments on pig marrow tissues were performed to further investigate the applicability of SGEN-J to biological samples with intrinsic susceptibility variations. Conclusion: Based on intrinsic advantages, SGEN-J serves as a helpful complement to existing 2D J-resolved methodologies in molecular structure elucidation and biomedical study, and offer bright perspectives for real-time analyzing in vivo biological systems and monitoring in situ chemical reactions.

Original languageEnglish (US)
Article number8490732
Pages (from-to)1559-1566
Number of pages8
JournalIEEE Transactions on Biomedical Engineering
Issue number6
StatePublished - Jun 2019


  • 2D homonuclear J-resolved spectra
  • NMR spectroscopy
  • biological detection
  • inhomogeneous magnetic fields
  • single scan

ASJC Scopus subject areas

  • Biomedical Engineering

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