A single mutation in the carboxy terminus of reovirus outer-capsid protein σ3 confers enhanced kinetics of σ3 proteolysis, resistance to inhibitors of viral disassembly, and alterations in σ3 structure

Gregory J. Wilson, Emma L. Nason, Charles S. Hardy, Daniel Ebert, J. Denise Wetzel, B. V. Venkataram Prasad, Terence S. Dermody

Research output: Contribution to journalArticle

Abstract

Mammalian reoviruses undergo acid-dependent proteolytic disassembly within endosomes, resulting in formation of infectious subvirion particles (ISVPs). ISVPs are obligate intermediates in reovirus disassembly that mediate viral penetration into the cytoplasm. The initial biochemical event in the reovirus disassembly pathway is the proteolysis of viral outer-capsid protein σ3. Mutant reoviruses selected during persistent infection of murine L929 cells (PI viruses) demonstrate enhanced kinetics of viral disassembly and resistance to inhibitors of endocytic acidification and proteolysis. To identify sequences in σ3 that modulate acid-dependent and protease-dependent steps in reovirus disassembly, the σ3 proteins of wild-type strain type 3 Dearing; PI viruses L/C, PI 2A1, and PI 3-1; and four novel mutant σ3 proteins were expressed in insect cells and used to recoat ISVPs. Treatment of recoated ISVPs (rISVPs) with either of the endocytic proteases cathepsin L or cathepsin D demonstrated that an isolated tyrosine-to-histidine mutation at amino acid 354 (Y354H) enhanced σ3 proteolysis during viral disassembly. Yields of rISVPs containing Y354H in σ3 were substantially greater than those of rISVPs lacking this mutation after growth in cells treated with either acidification inhibitor ammonium chloride or cysteine protease inhibitor E64. Image reconstructions of electron micrographs of virus particles containing wild-type or mutant σ3 proteins revealed structural alterations in σ3 that correlate with the Y354H mutation. These results indicate that a single mutation in σ3 protein alters its susceptibility to proteolysis and provide a structural framework to understand mechanisms of σ3 cleavage during reovirus disassembly.

Original languageEnglish (US)
Pages (from-to)9832-9843
Number of pages12
JournalJournal of Virology
Volume76
Issue number19
DOIs
StatePublished - Oct 2002
Externally publishedYes

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Reoviridae
Capsid Proteins
coat proteins
proteolysis
Proteolysis
mutation
kinetics
Mutation
Mutant Proteins
Peptide Hydrolases
Mammalian orthoreovirus 3
Viruses
Cysteine Proteinase Inhibitors
Cathepsin L
Ammonium Chloride
mutants
Cathepsin D
acidification
Acids
Computer-Assisted Image Processing

ASJC Scopus subject areas

  • Immunology

Cite this

A single mutation in the carboxy terminus of reovirus outer-capsid protein σ3 confers enhanced kinetics of σ3 proteolysis, resistance to inhibitors of viral disassembly, and alterations in σ3 structure. / Wilson, Gregory J.; Nason, Emma L.; Hardy, Charles S.; Ebert, Daniel; Wetzel, J. Denise; Venkataram Prasad, B. V.; Dermody, Terence S.

In: Journal of Virology, Vol. 76, No. 19, 10.2002, p. 9832-9843.

Research output: Contribution to journalArticle

Wilson, Gregory J. ; Nason, Emma L. ; Hardy, Charles S. ; Ebert, Daniel ; Wetzel, J. Denise ; Venkataram Prasad, B. V. ; Dermody, Terence S. / A single mutation in the carboxy terminus of reovirus outer-capsid protein σ3 confers enhanced kinetics of σ3 proteolysis, resistance to inhibitors of viral disassembly, and alterations in σ3 structure. In: Journal of Virology. 2002 ; Vol. 76, No. 19. pp. 9832-9843.
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abstract = "Mammalian reoviruses undergo acid-dependent proteolytic disassembly within endosomes, resulting in formation of infectious subvirion particles (ISVPs). ISVPs are obligate intermediates in reovirus disassembly that mediate viral penetration into the cytoplasm. The initial biochemical event in the reovirus disassembly pathway is the proteolysis of viral outer-capsid protein σ3. Mutant reoviruses selected during persistent infection of murine L929 cells (PI viruses) demonstrate enhanced kinetics of viral disassembly and resistance to inhibitors of endocytic acidification and proteolysis. To identify sequences in σ3 that modulate acid-dependent and protease-dependent steps in reovirus disassembly, the σ3 proteins of wild-type strain type 3 Dearing; PI viruses L/C, PI 2A1, and PI 3-1; and four novel mutant σ3 proteins were expressed in insect cells and used to recoat ISVPs. Treatment of recoated ISVPs (rISVPs) with either of the endocytic proteases cathepsin L or cathepsin D demonstrated that an isolated tyrosine-to-histidine mutation at amino acid 354 (Y354H) enhanced σ3 proteolysis during viral disassembly. Yields of rISVPs containing Y354H in σ3 were substantially greater than those of rISVPs lacking this mutation after growth in cells treated with either acidification inhibitor ammonium chloride or cysteine protease inhibitor E64. Image reconstructions of electron micrographs of virus particles containing wild-type or mutant σ3 proteins revealed structural alterations in σ3 that correlate with the Y354H mutation. These results indicate that a single mutation in σ3 protein alters its susceptibility to proteolysis and provide a structural framework to understand mechanisms of σ3 cleavage during reovirus disassembly.",
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AU - Wilson, Gregory J.

AU - Nason, Emma L.

AU - Hardy, Charles S.

AU - Ebert, Daniel

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AU - Venkataram Prasad, B. V.

AU - Dermody, Terence S.

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