A simplified system for generating recombinant adenoviruses

Tong Chuan He, Shibin Zhou, Luis T. Da Costa, Jian Yu, Kenneth W. Kinzler, Bert Vogelstein

Research output: Contribution to journalArticlepeer-review

Abstract

Recombinant adenoviruses provide a versatile system for gene expression studies and therapeutic applications. We report herein a strategy that simplifies the generation and production of such viruses. A recombinant adenoviral plasmid is generated with a minimum of enzymatic manipulations, using homologous recombination in bacteria rather than in eukaryotic cells. After transfections of such plasmids into a mammalian packaging cell line, vital production is conveniently followed with the aid of green fluorescent protein, encoded by a gene incorporated into the viral backbone. Homogeneous viruses can be obtained from this procedure without plaque purification. This system should expedite the process of generating and testing recombinant adenoviruses for a variety of purposes.

Original languageEnglish (US)
Pages (from-to)2509-2514
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume95
Issue number5
DOIs
StatePublished - Mar 3 1998

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'A simplified system for generating recombinant adenoviruses'. Together they form a unique fingerprint.

Cite this