A simplified system for generating recombinant adenoviruses

Tong Chuan He, Shibin Zhou, Luis T. Da Costa, Jian Yu, Kenneth W Kinzler, Bert Vogelstein

Research output: Contribution to journalArticle

Abstract

Recombinant adenoviruses provide a versatile system for gene expression studies and therapeutic applications. We report herein a strategy that simplifies the generation and production of such viruses. A recombinant adenoviral plasmid is generated with a minimum of enzymatic manipulations, using homologous recombination in bacteria rather than in eukaryotic cells. After transfections of such plasmids into a mammalian packaging cell line, vital production is conveniently followed with the aid of green fluorescent protein, encoded by a gene incorporated into the viral backbone. Homogeneous viruses can be obtained from this procedure without plaque purification. This system should expedite the process of generating and testing recombinant adenoviruses for a variety of purposes.

Original languageEnglish (US)
Pages (from-to)2509-2514
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume95
Issue number5
DOIs
StatePublished - Mar 3 1998

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Adenoviridae
Plasmids
Viruses
Homologous Recombination
Eukaryotic Cells
Product Packaging
Green Fluorescent Proteins
Transfection
Bacteria
Gene Expression
Cell Line
Genes
Therapeutics

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

A simplified system for generating recombinant adenoviruses. / He, Tong Chuan; Zhou, Shibin; Da Costa, Luis T.; Yu, Jian; Kinzler, Kenneth W; Vogelstein, Bert.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, No. 5, 03.03.1998, p. 2509-2514.

Research output: Contribution to journalArticle

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