TY - JOUR
T1 - A simplified method for the production of murine T-cell growth factor free of lectin
AU - Spiess, Paul J.
AU - Rosenberg, Steven A.
N1 - Funding Information:
Surgery Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20205, U.S.A.
PY - 1981/4/29
Y1 - 1981/4/29
N2 - A simple and rapid method for the production of TCGF free of lectin (LF-TCGF) is described. Murine splenocytes are exposed to 10-20 μg/ml Con A for 2 h before washing the cells and incubating them in medium for an additional 20 h. This procedure leads to the same titers of TCGF activity obtained when Con A is present throughout the incubation time. The resulting TCGF preparation is free of Con A as evidenced by its inability to activate fresh resting lymphoid cells and by measurements using [3H] Con A in tracer amounts. The LF-TCGF is capable of growing activated but not resting lymphocytes and can lead to greater than 50-fold increase in the generation of cytotoxic cells in in vitro sensitizations to alloantigens.
AB - A simple and rapid method for the production of TCGF free of lectin (LF-TCGF) is described. Murine splenocytes are exposed to 10-20 μg/ml Con A for 2 h before washing the cells and incubating them in medium for an additional 20 h. This procedure leads to the same titers of TCGF activity obtained when Con A is present throughout the incubation time. The resulting TCGF preparation is free of Con A as evidenced by its inability to activate fresh resting lymphoid cells and by measurements using [3H] Con A in tracer amounts. The LF-TCGF is capable of growing activated but not resting lymphocytes and can lead to greater than 50-fold increase in the generation of cytotoxic cells in in vitro sensitizations to alloantigens.
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U2 - 10.1016/0022-1759(81)90151-4
DO - 10.1016/0022-1759(81)90151-4
M3 - Article
C2 - 6972416
AN - SCOPUS:0019508256
SN - 0022-1759
VL - 42
SP - 213
EP - 222
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
IS - 2
ER -