A simple radiometric in vitro assay for acetylcholinesterase inhibitors

Tomas R. Guilarte; H. Donald Burns; Robert F. Dannals; Henry N. Wagner

doi:10.1002/jps.2600720123

A simple radiometric in vitro assay for acetylcholinesterase inhibitors

Tomas R. Guilarte, H. Donald Burns, Robert F. Dannals, Henry N. Wagner

School of Medicine

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

A radiometric method for screening acetylcholinesterase inhibitors has been described. The method is based on the production of [¹⁴C]carbon dioxide from the hydrolysis of acetylcholine. The inhibitory concentration at 50% (IC₅₀) values for several known acetylcholinesterase inhibitors were in agreement with literature values. The new radiometric method is simple, inexpensive, and has the potential for automation.

Original language	English (US)
Pages (from-to)	90-92
Number of pages	3
Journal	Journal of Pharmaceutical Sciences
Volume	72
Issue number	1
DOIs	https://doi.org/10.1002/jps.2600720123
State	Published - Jan 1983

Keywords

Acetylcholinesterase inhibitors—a simple radiometric in vitro assay
Radiometric method—in vitro assay for acetylcholinesterase inhibitors

ASJC Scopus subject areas

Pharmaceutical Science

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10.1002/jps.2600720123

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abstract = "A radiometric method for screening acetylcholinesterase inhibitors has been described. The method is based on the production of [14C]carbon dioxide from the hydrolysis of acetylcholine. The inhibitory concentration at 50% (IC50) values for several known acetylcholinesterase inhibitors were in agreement with literature values. The new radiometric method is simple, inexpensive, and has the potential for automation.",

keywords = "Acetylcholinesterase inhibitors—a simple radiometric in vitro assay, Radiometric method—in vitro assay for acetylcholinesterase inhibitors",

author = "Guilarte, {Tomas R.} and Burns, {H. Donald} and Dannals, {Robert F.} and Wagner, {Henry N.}",

year = "1983",

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AB - A radiometric method for screening acetylcholinesterase inhibitors has been described. The method is based on the production of [14C]carbon dioxide from the hydrolysis of acetylcholine. The inhibitory concentration at 50% (IC50) values for several known acetylcholinesterase inhibitors were in agreement with literature values. The new radiometric method is simple, inexpensive, and has the potential for automation.

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A simple radiometric in vitro assay for acetylcholinesterase inhibitors

Abstract

Keywords

ASJC Scopus subject areas

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