A simple method of producing a monolayer of cervical cells for digital image processing

Dorothy Rosenthal, E. Stern, C. McLatchie, A. Wu, L. D. Lagasse, R. Wall, K. R. Castleman

Research output: Contribution to journalArticle

Abstract

A manual method of producing a monolayer of isolated and evenly distributed cervical epithelial cells has been developed for use in an automated digital image analysis system. The cervical scrape specimen is vortexed from the spatula into a solution of Mucosol. The suspension is filtered through a 10μ nylon mesh to remove polys and debris and then syringed to disaggregate epithelial clumps. The cells are plated out on a Nuclepore filter. The filter is touched directly onto a glass microscopic slide to transfer cells, which are then fixed and stained in a routine manner. Cell counts reveal elimination of 92% polys, with only 2% loss of squamous cells.

Original languageEnglish (US)
Pages (from-to)84-88
Number of pages5
JournalAnalytical and Quantitative Cytology
Volume1
Issue number2
StatePublished - 1979
Externally publishedYes

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Epithelial Cells
Nylons
Acetylcysteine
Glass
Suspensions
Cell Count

ASJC Scopus subject areas

  • Anatomy
  • Histology

Cite this

Rosenthal, D., Stern, E., McLatchie, C., Wu, A., Lagasse, L. D., Wall, R., & Castleman, K. R. (1979). A simple method of producing a monolayer of cervical cells for digital image processing. Analytical and Quantitative Cytology, 1(2), 84-88.

A simple method of producing a monolayer of cervical cells for digital image processing. / Rosenthal, Dorothy; Stern, E.; McLatchie, C.; Wu, A.; Lagasse, L. D.; Wall, R.; Castleman, K. R.

In: Analytical and Quantitative Cytology, Vol. 1, No. 2, 1979, p. 84-88.

Research output: Contribution to journalArticle

Rosenthal, D, Stern, E, McLatchie, C, Wu, A, Lagasse, LD, Wall, R & Castleman, KR 1979, 'A simple method of producing a monolayer of cervical cells for digital image processing', Analytical and Quantitative Cytology, vol. 1, no. 2, pp. 84-88.
Rosenthal, Dorothy ; Stern, E. ; McLatchie, C. ; Wu, A. ; Lagasse, L. D. ; Wall, R. ; Castleman, K. R. / A simple method of producing a monolayer of cervical cells for digital image processing. In: Analytical and Quantitative Cytology. 1979 ; Vol. 1, No. 2. pp. 84-88.
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