A simple method of producing a monolayer of cervical cells for digital image processing

A manual method of producing a monolayer of isolated and evenly distributed cervical epithelial cells has been developed for use in an automated digital image analysis system. The cervical scrape specimen is vortexed from the spatula into a solution of Mucosol. The suspension is filtered through a 10μ nylon mesh to remove polys and debris and then syringed to disaggregate epithelial clumps. The cells are plated out on a Nuclepore filter. The filter is touched directly onto a glass microscopic slide to transfer cells, which are then fixed and stained in a routine manner. Cell counts reveal elimination of 92% polys, with only 2% loss of squamous cells.