An enzyme immunoassay (EIA) system has been developed to measure factor VIII-related antigen VIII(AGN)). This assay gives similar results to the commonly used Laurell electroimmunodiffusion (EID) assay for VIII(AGN) as shown by comparison of both techniques with samples from healthy controls, patients with hemophilia A, and patients with von Willebrand's disease. The assay also has a greater precision that the EID technique as demonstrated by multiple assays of aliquots of a single sample. The use of this EIA test for VIII(AGN) is simple and employs inexpensive reagents and equipment. The use of expensive antisera is minimized. EIA for VIII(AGN) has the advantage of increased sensitivity compared to Laurell EIA.
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