A simple and rapid technique for the detection of rifampin resistance in Mycobacterium leprae

N. Honore, E. Perrani, A. Telenti, J. Grosset, S. T. Cole

Research output: Contribution to journalArticlepeer-review

Abstract

The rifampin resistance of Mycobacterium leprae is due to missense mutations in the rpoB gene encoding the β-subunit of the essential enzyme RNA polymerase. A rapid and very simple method has been developed to detect rifampin resistance in small numbers of M. leprae present in biopsies. It involves polymerase chain reaction amplification of a defined region of the rpoB gene followed by single-strand conformational polymorphism analysis (PCR-SSCP). The reliability of the method has been tested on a sample of known drug-resistant and -susceptible isolates of M. leprae.

Original languageEnglish (US)
Pages (from-to)600-604
Number of pages5
JournalInternational Journal of Leprosy
Volume61
Issue number4
StatePublished - Dec 1 1993

ASJC Scopus subject areas

  • Dermatology

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