Abstract
The rifampin resistance of Mycobacterium leprae is due to missense mutations in the rpoB gene encoding the β-subunit of the essential enzyme RNA polymerase. A rapid and very simple method has been developed to detect rifampin resistance in small numbers of M. leprae present in biopsies. It involves polymerase chain reaction amplification of a defined region of the rpoB gene followed by single-strand conformational polymorphism analysis (PCR-SSCP). The reliability of the method has been tested on a sample of known drug-resistant and -susceptible isolates of M. leprae.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 600-604 |
| Number of pages | 5 |
| Journal | International Journal of Leprosy |
| Volume | 61 |
| Issue number | 4 |
| State | Published - 1993 |
| Externally published | Yes |
ASJC Scopus subject areas
- Dermatology