A Simple and Effective Method for Flow Cytometric Study of Lymphoid Malignancies Using Needle Core Biopsy Specimens

Pei Dong Chi, Natalie S. Freed, Laura Wake, Cynthia Page, Lynette M. Smith, Catalina Amador, Hina Naushad Qureishi, Timothy C. Greiner, Scott J. Rodig, Samuel J. Pirruccello, Ji Yuan, Kai Fu

Research output: Contribution to journalArticle

Abstract

Objectives: We developed a simple and effective rinsing technique (RT) of needle biopsies to produce cell suspensions for flow cytometry (FCM) and evaluated whether the RT is comparable to the conventional tissue cell suspension (TCS) technique. Methods: We retrieved 93 needle core biopsy cases employing the RT for FCM and 25 needle biopsy cases using TCS for FCM. Results: The diagnostic concordance between the FCM results and the morphologic diagnoses of both groups was compared. The diagnostic concordance was comparable in the RT group (92.6%) to the TCS group (71.4%). Furthermore, the diagnostic concordance in the RT group was associated with number of isolated cells. The diagnostic accuracy increased significantly when the cell number was above 30,000 in the RT group. Conclusions: The RT for FCM not only maximizes the tissue utilization, but also is a simple and effective method to obtain cell suspension as compared to traditional cell suspension technique.

Original languageEnglish (US)
JournalCytometry Part B - Clinical Cytometry
DOIs
StateAccepted/In press - Jan 1 2018
Externally publishedYes

Fingerprint

Large-Core Needle Biopsy
Suspensions
Flow Cytometry
Neoplasms
Needle Biopsy
Cell Count

Keywords

  • Flow cytometry
  • Lymphoma
  • Needle core biopsy
  • Pathologic diagnosis

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

Cite this

A Simple and Effective Method for Flow Cytometric Study of Lymphoid Malignancies Using Needle Core Biopsy Specimens. / Chi, Pei Dong; Freed, Natalie S.; Wake, Laura; Page, Cynthia; Smith, Lynette M.; Amador, Catalina; Qureishi, Hina Naushad; Greiner, Timothy C.; Rodig, Scott J.; Pirruccello, Samuel J.; Yuan, Ji; Fu, Kai.

In: Cytometry Part B - Clinical Cytometry, 01.01.2018.

Research output: Contribution to journalArticle

Chi, PD, Freed, NS, Wake, L, Page, C, Smith, LM, Amador, C, Qureishi, HN, Greiner, TC, Rodig, SJ, Pirruccello, SJ, Yuan, J & Fu, K 2018, 'A Simple and Effective Method for Flow Cytometric Study of Lymphoid Malignancies Using Needle Core Biopsy Specimens', Cytometry Part B - Clinical Cytometry. https://doi.org/10.1002/cyto.b.21634
Chi, Pei Dong ; Freed, Natalie S. ; Wake, Laura ; Page, Cynthia ; Smith, Lynette M. ; Amador, Catalina ; Qureishi, Hina Naushad ; Greiner, Timothy C. ; Rodig, Scott J. ; Pirruccello, Samuel J. ; Yuan, Ji ; Fu, Kai. / A Simple and Effective Method for Flow Cytometric Study of Lymphoid Malignancies Using Needle Core Biopsy Specimens. In: Cytometry Part B - Clinical Cytometry. 2018.
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abstract = "Objectives: We developed a simple and effective rinsing technique (RT) of needle biopsies to produce cell suspensions for flow cytometry (FCM) and evaluated whether the RT is comparable to the conventional tissue cell suspension (TCS) technique. Methods: We retrieved 93 needle core biopsy cases employing the RT for FCM and 25 needle biopsy cases using TCS for FCM. Results: The diagnostic concordance between the FCM results and the morphologic diagnoses of both groups was compared. The diagnostic concordance was comparable in the RT group (92.6{\%}) to the TCS group (71.4{\%}). Furthermore, the diagnostic concordance in the RT group was associated with number of isolated cells. The diagnostic accuracy increased significantly when the cell number was above 30,000 in the RT group. Conclusions: The RT for FCM not only maximizes the tissue utilization, but also is a simple and effective method to obtain cell suspension as compared to traditional cell suspension technique.",
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AU - Page, Cynthia

AU - Smith, Lynette M.

AU - Amador, Catalina

AU - Qureishi, Hina Naushad

AU - Greiner, Timothy C.

AU - Rodig, Scott J.

AU - Pirruccello, Samuel J.

AU - Yuan, Ji

AU - Fu, Kai

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KW - Lymphoma

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