A short CIC-2 mRNA transcript is produced by exon skipping

Shijian Chu, Carol B. Murray, Minzhi M. Liu, Pamela L. Zeitlin

Research output: Contribution to journalArticle

Abstract

CIC-2 is a voltage- and volume-regulated chloride channel expressed in many tissues. We have shown that CIC-2 in rat lung airways is significantly down-regulated after birth. During PCR amplification from rat lung cDNA, a second transcript was identified which is 60 bp shorter than the full length sequence. The peptide translated from this 60 bp sequence contains many positively charged amino acid residues. Rat genomic DNA sequencing showed that the 60 bp sequence is an intact exon. A 71% pyrimidine content and an AAG 3'-end splice site in the intron immediately upstream from the 60 bp sequence were identified which may account for the alternative splicing of the following exon. Human genomic sequence analyses demonstrated similar intron-exon arrangement. A high CT content and an AAG 3' acceptor site were conserved in the intron corresponding to the rat upstream intron. The presence of the full length short form transcript was confirmed in rat kidney by RT-PCR, and the ratio of the long and the short form transcripts varied significantly according to the tissues examined, with the lowest long/short form ratio found in the lung among the tissues studied. Our data demonstrated that the alternatively spliced short form (CIC-2S) is transcribed in many rat tissues, the ratio of the long/short form transcripts is lower in the lung compared with the brain, and the genomic organization in this area is conserved in rat and human.

Original languageEnglish (US)
Pages (from-to)3453-3457
Number of pages5
JournalNucleic Acids Research
Volume24
Issue number17
StatePublished - 1996

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Exons
Messenger RNA
Introns
Lung
Polymerase Chain Reaction
RNA Splice Sites
Chloride Channels
Alternative Splicing
DNA Sequence Analysis
Sequence Analysis
Complementary DNA
Parturition
Kidney
Amino Acids
Peptides
Brain

ASJC Scopus subject areas

  • Genetics

Cite this

Chu, S., Murray, C. B., Liu, M. M., & Zeitlin, P. L. (1996). A short CIC-2 mRNA transcript is produced by exon skipping. Nucleic Acids Research, 24(17), 3453-3457.

A short CIC-2 mRNA transcript is produced by exon skipping. / Chu, Shijian; Murray, Carol B.; Liu, Minzhi M.; Zeitlin, Pamela L.

In: Nucleic Acids Research, Vol. 24, No. 17, 1996, p. 3453-3457.

Research output: Contribution to journalArticle

Chu, S, Murray, CB, Liu, MM & Zeitlin, PL 1996, 'A short CIC-2 mRNA transcript is produced by exon skipping', Nucleic Acids Research, vol. 24, no. 17, pp. 3453-3457.
Chu S, Murray CB, Liu MM, Zeitlin PL. A short CIC-2 mRNA transcript is produced by exon skipping. Nucleic Acids Research. 1996;24(17):3453-3457.
Chu, Shijian ; Murray, Carol B. ; Liu, Minzhi M. ; Zeitlin, Pamela L. / A short CIC-2 mRNA transcript is produced by exon skipping. In: Nucleic Acids Research. 1996 ; Vol. 24, No. 17. pp. 3453-3457.
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