A serum miRNA profile of human longevity: Findings from the Baltimore Longitudinal Study of Aging (BLSA)

Thalyana Smith-Vikos, Zuyun Liu, Christine Parsons, Myriam Gorospe, Luigi Ferrucci, Thomas M. Gill, Frank J. Slack

Research output: Contribution to journalArticlepeer-review

Abstract

In C. elegans, miRNAs are genetic biomarkers of aging. Similarly, multiple miRNAs are differentially expressed between younger and older persons, suggesting that miRNA-regulated biological mechanisms affecting aging are evolutionarily conserved. Previous human studies have not considered participants' lifespans, a key factor in identifying biomarkers of aging. Using PCR arrays, we measured miRNA levels from serum samples obtained longitudinally at ages 50, 55, and 60 from 16 non-Hispanic males who had documented lifespans from 58 to 92. Numerous miRNAs showed significant changes in expression levels. At age 50, 24 miRNAs were significantly upregulated, and 73 were significantly downregulated in the long-lived subgroup (76-92 years) as compared with the short-lived subgroup (58-75 years). In long-lived participants, the most upregulated was miR-373-5p, while the most downregulated was miR-15b-5p. Longitudinally, significant Pearson correlations were observed between lifespan and expression of nine miRNAs (p value < 0.05). Six of these nine miRNAs (miR-211-5p, 374a-5p, 340-3p, 376c-3p, 5095, 1225-3p) were also significantly up- or downregulated when comparing long-lived and short-lived participants. Twenty-four validated targets of these miRNAs encoded aging-associated proteins, including PARP1, IGF1R, and IGF2R. We propose that the expression profiles of the six miRNAs (miR-211-5p, 374a-5p, 340-3p, 376c-3p, 5095, and 1225-3p) may be useful biomarkers of aging.

Original languageEnglish (US)
Pages (from-to)2971-2987
Number of pages17
JournalAging
Volume8
Issue number11
DOIs
StatePublished - 2016
Externally publishedYes

Keywords

  • Aging
  • Biomarker
  • Long-lived
  • Longitudinal study
  • MiRNA
  • Short-lived

ASJC Scopus subject areas

  • Aging
  • Cell Biology

Fingerprint Dive into the research topics of 'A serum miRNA profile of human longevity: Findings from the Baltimore Longitudinal Study of Aging (BLSA)'. Together they form a unique fingerprint.

Cite this