A SENSITIVE SPECIFIC ENZYMATIC‐FLUOROMETRIC ASSAY FOR HOMOCARNOSINE

Anne B. Young, S. H. Snyder

Research output: Contribution to journalArticle

Abstract

We have developed a sensitive and specific assay for homocarnosine in tissues. Homocarnosine is separated from GABA by ion exchange chromatography. After hydrolysis of homocarnosine with swine kidney carnosinase, the evolved GABA is measured by an enzymatic‐fluorometric procedure. As little as 0.1 nmol of tissue homocarnosine can be detected by this procedure. Homoanserine, which would be detected by this assay, can be separated from homocarnosine by thin layer chromatography. No homoanserine could be detected in any tissues examined. There are marked regional variations in levels of homocarnosine in guinea‐pig brain that do not correspond to regional differences in GABA levels.

Original languageEnglish (US)
Pages (from-to)387-396
Number of pages10
JournalJournal of Neurochemistry
Volume21
Issue number2
DOIs
StatePublished - Aug 1973

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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