TY - JOUR
T1 - A sensitive rosetting assay for detection of acetylcholine receptor antibodies using BC3H-1 cells
T2 - positive results in 'antibody-negative' myasthenia gravis
AU - Brooks, Elizabeth B.
AU - Pachner, Andrew R.
AU - Drachman, Daniel B.
AU - Kantor, Fred S.
N1 - Funding Information:
We thank Drs. Robert Brown and Joseph Craft for donating sera from their patients and Dr. David Richman for advice on extraction of mammalian muscle. We also thank the Mayo Medical Laboratory (Dr. Vanda Lennon) for performing the blocking anti-AChR and anti-striational antibody determinations. This work was supported by National Institutes of Health Grant AI17716 and 5ROIHD04817, U.S. Public Health Service Training Grant 5T32NS07036-09,10,10(EXT), and grants from the Muscular Dystrophy Association and the Myasthenia Gravis Foundation (Potomac Valley Chapter). This paper is based on thesis work done by Dr. Brooks to satisfy the requirements for the PhD at Yale University in the Department of Epidemiology and Public Health.
PY - 1990/6
Y1 - 1990/6
N2 - Antibodies to acetylcholine receptor (AChR) were measured in a group of patients with myasthenia gravis (MG), some of whom had previously been classified as "antibody negative" using the standard anti-AChR radioimmunoassay (RIA). AChR antibodies were measured using the resotting assay, a new detection method which utilizes protein A-coated red blood cells and live BC3H-1 cells, a murine cell line which expresses muscle nicotinic AChR. The results of the rosetting assay were compared with those obtained in the anti-AChR RIA. 76% of all myasthenic sera tested showed rosetting at titers higher than any of the control sera (from patients with non-myasthenic neurologic disease and normal individuals). Of the myasthenic patients previously classified as 'antibody negative' in the RIA using human AChR, 71% demonstrated positive rosetting. There was no correlation between the anti-AChR antibody titer obtained in the rosetting assay and that obtained in the RIA using either human or denervated rat AChR. The results suggest that the rosetting assay may meausure a subpopulation of antibodies that differs from those detected in the RIA.
AB - Antibodies to acetylcholine receptor (AChR) were measured in a group of patients with myasthenia gravis (MG), some of whom had previously been classified as "antibody negative" using the standard anti-AChR radioimmunoassay (RIA). AChR antibodies were measured using the resotting assay, a new detection method which utilizes protein A-coated red blood cells and live BC3H-1 cells, a murine cell line which expresses muscle nicotinic AChR. The results of the rosetting assay were compared with those obtained in the anti-AChR RIA. 76% of all myasthenic sera tested showed rosetting at titers higher than any of the control sera (from patients with non-myasthenic neurologic disease and normal individuals). Of the myasthenic patients previously classified as 'antibody negative' in the RIA using human AChR, 71% demonstrated positive rosetting. There was no correlation between the anti-AChR antibody titer obtained in the rosetting assay and that obtained in the RIA using either human or denervated rat AChR. The results suggest that the rosetting assay may meausure a subpopulation of antibodies that differs from those detected in the RIA.
KW - Acetylcholine receptor antibody
KW - Myasthenia gravis, antibody-negative
KW - Rosetting assay
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U2 - 10.1016/0165-5728(90)90043-M
DO - 10.1016/0165-5728(90)90043-M
M3 - Article
C2 - 2341562
AN - SCOPUS:0025361464
SN - 0165-5728
VL - 28
SP - 83
EP - 93
JO - Journal of Neuroimmunology
JF - Journal of Neuroimmunology
IS - 1
ER -