A sensitive non-radioactive assay for pyrimidine nucleoside phosphorylase using reversed-phase high performance liquid chromatography

Emile J. Laurensse; Herbert M. Pinedo; Godefridus J. Peters

doi:10.1016/0009-8981(88)90270-7

A sensitive non-radioactive assay for pyrimidine nucleoside phosphorylase using reversed-phase high performance liquid chromatography

Emile J. Laurensse, Herbert M. Pinedo, Godefridus J. Peters

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

A new sensitive assay for pyrimidine nucleoside phosphorylase using non-radioactive substrates is described. With the natural substrate undine (UR) and the analog, 5′-deoxy-5-fluorouridine (5′dFUR) conditions have been optimized to measure the product formation with reversed-phase high-performance liquid chromatography. Using automated injection large series of samples may be analyzed. The assay for UR phosphorylase appeared to be comparable to existing methods with radiolabeled UR as substrate regarding sensitivity and linearity. The assay has been used to measure kinetic parameters for 5′dFUR and UR in two cell lines from intestinal origin.

Original language	English (US)
Pages (from-to)	71-78
Number of pages	8
Journal	Clinica Chimica Acta
Volume	178
Issue number	1
DOIs	https://doi.org/10.1016/0009-8981(88)90270-7
State	Published - 1988
Externally published	Yes

Keywords

5′-Deoxy-5-fluorouridine
High-performance liquid chromatography
Pyrimidine nucleoside phosphorylase
Uridine

ASJC Scopus subject areas

Biochemistry
Clinical Biochemistry

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10.1016/0009-8981(88)90270-7

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title = "A sensitive non-radioactive assay for pyrimidine nucleoside phosphorylase using reversed-phase high performance liquid chromatography",

abstract = "A new sensitive assay for pyrimidine nucleoside phosphorylase using non-radioactive substrates is described. With the natural substrate undine (UR) and the analog, 5′-deoxy-5-fluorouridine (5′dFUR) conditions have been optimized to measure the product formation with reversed-phase high-performance liquid chromatography. Using automated injection large series of samples may be analyzed. The assay for UR phosphorylase appeared to be comparable to existing methods with radiolabeled UR as substrate regarding sensitivity and linearity. The assay has been used to measure kinetic parameters for 5′dFUR and UR in two cell lines from intestinal origin.",

keywords = "5′-Deoxy-5-fluorouridine, High-performance liquid chromatography, Pyrimidine nucleoside phosphorylase, Uridine",

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T1 - A sensitive non-radioactive assay for pyrimidine nucleoside phosphorylase using reversed-phase high performance liquid chromatography

AU - Laurensse, Emile J.

AU - Pinedo, Herbert M.

AU - Peters, Godefridus J.

PY - 1988

Y1 - 1988

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AB - A new sensitive assay for pyrimidine nucleoside phosphorylase using non-radioactive substrates is described. With the natural substrate undine (UR) and the analog, 5′-deoxy-5-fluorouridine (5′dFUR) conditions have been optimized to measure the product formation with reversed-phase high-performance liquid chromatography. Using automated injection large series of samples may be analyzed. The assay for UR phosphorylase appeared to be comparable to existing methods with radiolabeled UR as substrate regarding sensitivity and linearity. The assay has been used to measure kinetic parameters for 5′dFUR and UR in two cell lines from intestinal origin.

KW - 5′-Deoxy-5-fluorouridine

KW - High-performance liquid chromatography

KW - Pyrimidine nucleoside phosphorylase

KW - Uridine

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A sensitive non-radioactive assay for pyrimidine nucleoside phosphorylase using reversed-phase high performance liquid chromatography

Abstract

Keywords

ASJC Scopus subject areas

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