A sensitive assay for quantitating 'unscheduled DNA synthesis' (repair synthesis) in transformed human amnion (AV3) cells has been developed. The combined use of hydroxyurea and arginine-deficient culture medium enabled the detection of 10-20 fold increases in 'unscheduled DNA synthesis' after treatment with N-acetoxy-2-acetylaminofluorene or ultraviolet light. The technique allows the detection of 'DNA repair synthesis' following treatment with extremely low doses of mutagens and carcinogens.
ASJC Scopus subject areas
- Cell Biology