Abstract
A sensitive assay for quantitating 'unscheduled DNA synthesis' (repair synthesis) in transformed human amnion (AV3) cells has been developed. The combined use of hydroxyurea and arginine-deficient culture medium enabled the detection of 10-20 fold increases in 'unscheduled DNA synthesis' after treatment with N-acetoxy-2-acetylaminofluorene or ultraviolet light. The technique allows the detection of 'DNA repair synthesis' following treatment with extremely low doses of mutagens and carcinogens.
Original language | English (US) |
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Pages (from-to) | 47-55 |
Number of pages | 9 |
Journal | Experimental cell research |
Volume | 88 |
Issue number | 1 |
DOIs | |
State | Published - Sep 1974 |
Externally published | Yes |
ASJC Scopus subject areas
- Cell Biology