A sensitive method to measure physical and chemical carcinogen-induced "unscheduled DNA synthesis" in rapidly dividing eukaryotic cells

J. E. Trosko, J. D. Yager

Research output: Contribution to journalArticle

Abstract

A sensitive assay for quantitating 'unscheduled DNA synthesis' (repair synthesis) in transformed human amnion (AV3) cells has been developed. The combined use of hydroxyurea and arginine-deficient culture medium enabled the detection of 10-20 fold increases in 'unscheduled DNA synthesis' after treatment with N-acetoxy-2-acetylaminofluorene or ultraviolet light. The technique allows the detection of 'DNA repair synthesis' following treatment with extremely low doses of mutagens and carcinogens.

Original languageEnglish (US)
Pages (from-to)47-55
Number of pages9
JournalExperimental cell research
Volume88
Issue number1
DOIs
StatePublished - Sep 1974
Externally publishedYes

ASJC Scopus subject areas

  • Cell Biology

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