A sensitive high-performance liquid chromatographic method for determination of the anti-neoplastic agents vp 16-213 and vm 26 in biological fluids

J. J M Holthuis, W. J. Van Oort, H. M. Pinedo

Research output: Contribution to journalArticle

Abstract

A rapid and sensitive high-performance liquid chromatographic assay of the two antineoplastic podophyllotoxin analogs VP 16-213 and VM 26 in plasma and urine is described. The drugs are extracted, after adding an internal standard (ethyl ester of p-hydroxybenzoic acid) with an optimum amount of chloroform. After evaporation of the organic layer, the extracts are redissolved in the eluent and chromatographed on a Lichrosorb reversedphase C15 column, with u.v. detection at 280 nm. Quantification is based on peak height ratios. The combination of an improved sample treatment and proper selection of chromatographic conditions results in a limit of determination of 30 ng of VP 16-213 or 50 ng of VM 26 per ml of plasma. Preliminary clinical pharmacokinetic results show that the sensitivity and selectivity of the assay are adequate for drug monitoring in clinical research.

Original languageEnglish (US)
Pages (from-to)23-30
Number of pages8
JournalAnalytica Chimica Acta
Volume130
Issue number1
DOIs
StatePublished - Sep 1 1981
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry
  • Spectroscopy
  • Environmental Chemistry

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