TY - JOUR
T1 - A sensitive high-performance liquid chromatographic method for determination of the anti-neoplastic agents vp 16-213 and vm 26 in biological fluids
AU - Holthuis, J. J M
AU - Van Oort, W. J.
AU - Pinedo, H. M.
PY - 1981/9/1
Y1 - 1981/9/1
N2 - A rapid and sensitive high-performance liquid chromatographic assay of the two antineoplastic podophyllotoxin analogs VP 16-213 and VM 26 in plasma and urine is described. The drugs are extracted, after adding an internal standard (ethyl ester of p-hydroxybenzoic acid) with an optimum amount of chloroform. After evaporation of the organic layer, the extracts are redissolved in the eluent and chromatographed on a Lichrosorb reversedphase C15 column, with u.v. detection at 280 nm. Quantification is based on peak height ratios. The combination of an improved sample treatment and proper selection of chromatographic conditions results in a limit of determination of 30 ng of VP 16-213 or 50 ng of VM 26 per ml of plasma. Preliminary clinical pharmacokinetic results show that the sensitivity and selectivity of the assay are adequate for drug monitoring in clinical research.
AB - A rapid and sensitive high-performance liquid chromatographic assay of the two antineoplastic podophyllotoxin analogs VP 16-213 and VM 26 in plasma and urine is described. The drugs are extracted, after adding an internal standard (ethyl ester of p-hydroxybenzoic acid) with an optimum amount of chloroform. After evaporation of the organic layer, the extracts are redissolved in the eluent and chromatographed on a Lichrosorb reversedphase C15 column, with u.v. detection at 280 nm. Quantification is based on peak height ratios. The combination of an improved sample treatment and proper selection of chromatographic conditions results in a limit of determination of 30 ng of VP 16-213 or 50 ng of VM 26 per ml of plasma. Preliminary clinical pharmacokinetic results show that the sensitivity and selectivity of the assay are adequate for drug monitoring in clinical research.
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U2 - 10.1016/S0003-2670(01)84147-7
DO - 10.1016/S0003-2670(01)84147-7
M3 - Article
AN - SCOPUS:0019416811
SN - 0003-2670
VL - 130
SP - 23
EP - 30
JO - Analytica Chimica Acta
JF - Analytica Chimica Acta
IS - 1
ER -