A sensitive gel filtration method for determination of protein sulfhydryl groups with 14C-chloromercuribenzoate

V. Gene Erwin, Peter L. Pedersen

Research output: Contribution to journalArticlepeer-review

Abstract

A gel filtration method employing 14C-chloromercuribenzoic acid is described for the quantitative determination of sulfhydryl groups in microgram quantities of protein. The method has been applied to several native proteins, hemoglobin, monoamine oxidase, and yeast cytochrome c. In all cases values in close agreement with known literature values were obtained. Horse heart cytochrome c and lysozyme, which have no sulfhydryl groups, did not bind the mercurial reagent. Modifications of the method are described for determining the sulfhydryl content of denatured proteins in the presence of sodium lauryl sulfate. The precision of the method was found to be compatible with known methods for determining the sulfhydryl composition of proteins.

Original languageEnglish (US)
Pages (from-to)477-485
Number of pages9
JournalAnalytical biochemistry
Volume25
Issue numberC
DOIs
StatePublished - 1968

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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