A sensitive and specific fluorescence assay for tissue serotonin

Solomon H. Snyder; Julius Axelrod; Mark Zweig

doi:10.1016/0006-2952(65)90102-4

A sensitive and specific fluorescence assay for tissue serotonin

Solomon H. Snyder, Julius Axelrod, Mark Zweig

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Abstract

A sensitive and specific method for the estimation of serotonin in biological materials is described. In this method, serotonin is reacted with ninhydrin to form a product whose fluorescence is eight times more intense than the native fluorescence of serotonin in strong acid solution. With this method it is possible to measure serotonin in organs in which endogenous serotonin had not been previously detected and to study the subcellular distribution of this amine in the rat pineal and adrenal glands.

Original language	English (US)
Pages (from-to)	831-835
Number of pages	5
Journal	Biochemical Pharmacology
Volume	14
Issue number	5
DOIs	https://doi.org/10.1016/0006-2952(65)90102-4
State	Published - May 1965
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Pharmacology

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10.1016/0006-2952(65)90102-4

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title = "A sensitive and specific fluorescence assay for tissue serotonin",

abstract = "A sensitive and specific method for the estimation of serotonin in biological materials is described. In this method, serotonin is reacted with ninhydrin to form a product whose fluorescence is eight times more intense than the native fluorescence of serotonin in strong acid solution. With this method it is possible to measure serotonin in organs in which endogenous serotonin had not been previously detected and to study the subcellular distribution of this amine in the rat pineal and adrenal glands.",

author = "Snyder, {Solomon H.} and Julius Axelrod and Mark Zweig",

year = "1965",

month = may,

doi = "10.1016/0006-2952(65)90102-4",

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journal = "Biochemical Pharmacology",

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T1 - A sensitive and specific fluorescence assay for tissue serotonin

AU - Snyder, Solomon H.

AU - Axelrod, Julius

AU - Zweig, Mark

PY - 1965/5

Y1 - 1965/5

N2 - A sensitive and specific method for the estimation of serotonin in biological materials is described. In this method, serotonin is reacted with ninhydrin to form a product whose fluorescence is eight times more intense than the native fluorescence of serotonin in strong acid solution. With this method it is possible to measure serotonin in organs in which endogenous serotonin had not been previously detected and to study the subcellular distribution of this amine in the rat pineal and adrenal glands.

AB - A sensitive and specific method for the estimation of serotonin in biological materials is described. In this method, serotonin is reacted with ninhydrin to form a product whose fluorescence is eight times more intense than the native fluorescence of serotonin in strong acid solution. With this method it is possible to measure serotonin in organs in which endogenous serotonin had not been previously detected and to study the subcellular distribution of this amine in the rat pineal and adrenal glands.

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U2 - 10.1016/0006-2952(65)90102-4

DO - 10.1016/0006-2952(65)90102-4

M3 - Article

C2 - 5840731

AN - SCOPUS:0013781730

SN - 0006-2952

VL - 14

SP - 831

EP - 835

JO - Biochemical Pharmacology

JF - Biochemical Pharmacology

IS - 5

ER -

A sensitive and specific fluorescence assay for tissue serotonin

Abstract

ASJC Scopus subject areas

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