TY - JOUR
T1 - A screen for non-coding RNA in Mycobacterium tuberculosis reveals a cAMP-responsive RNA that is expressed during infection
AU - Pelly, Shaaretha
AU - Bishai, William R.
AU - Lamichhane, Gyanu
N1 - Funding Information:
We thank Hani Zaher for expert advice on RNA related studies, Maia Schoonmaker for providing RNA from infected animals and Cara Smith for assistance with cDNA library screening and dot-blot analysis. A gift of the miRCAT™ RNA cloning Kit from Integrated DNA Technologies (IDT) is appreciated. This work was funded by NIH/NIAID AI30036 , AI079590 , AI37856 , AI36973 and DP2OD008459 . SP, WRB and GL planned the studies, SP conducted them, SP, WRB and GL analyzed the data and SP and GL wrote the manuscript.
PY - 2012/5/25
Y1 - 2012/5/25
N2 - A key to the success of Mycobacterium tuberculosis (Mtb) is the bacteria's ability to survive and thrive in the presence of numerous stresses mounted by the host. Small, non-coding RNAs (sRNAs) have been shown to modulate numerous stress responses in bacteria, yet to date only two studies have screened the Mtb transcriptome to identify sRNA. Their association with oxidative and acid stress has been demonstrated but the cellular function and role of these sRNAs in the pathogenesis of tuberculosis (TB) remain unknown. Here, we have identified an sRNA, ncrMT1302, in a locus involved in cAMP metabolism and demonstrate that expression of ncrMT1302 responds to changes in pH and cAMP concentration. The differential expression of ncrMT1302 observed in wild-type Mtb during growth is abolished in a strain lacking MT1302, an adenylyl cyclase encoding gene. We report that ncrMT1302 is expressed in Mtb residing in the lungs of mice during an active infection.
AB - A key to the success of Mycobacterium tuberculosis (Mtb) is the bacteria's ability to survive and thrive in the presence of numerous stresses mounted by the host. Small, non-coding RNAs (sRNAs) have been shown to modulate numerous stress responses in bacteria, yet to date only two studies have screened the Mtb transcriptome to identify sRNA. Their association with oxidative and acid stress has been demonstrated but the cellular function and role of these sRNAs in the pathogenesis of tuberculosis (TB) remain unknown. Here, we have identified an sRNA, ncrMT1302, in a locus involved in cAMP metabolism and demonstrate that expression of ncrMT1302 responds to changes in pH and cAMP concentration. The differential expression of ncrMT1302 observed in wild-type Mtb during growth is abolished in a strain lacking MT1302, an adenylyl cyclase encoding gene. We report that ncrMT1302 is expressed in Mtb residing in the lungs of mice during an active infection.
KW - Acid stress
KW - Small regulatory RNA
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U2 - 10.1016/j.gene.2012.03.044
DO - 10.1016/j.gene.2012.03.044
M3 - Article
C2 - 22446041
AN - SCOPUS:84860349834
SN - 0378-1119
VL - 500
SP - 85
EP - 92
JO - Gene
JF - Gene
IS - 1
ER -