Abstract
A large proportion of ongoing malaria parasite transmission is attributed to low-density subclinical infections not readily detected by available rapid diagnostic tests (RDTs) or microscopy. Plasmodium falciparum gametocyte carriage is subclinical, but gametocytemic individuals comprise the parasite reservoir that leads to infection of mosquitoes and local transmission. Effective detection and quantification of these carriers can help advance malaria elimination strategies. However, no point-of-need (PON) RDTs for gametocyte detection exist, much less one that can perform noninvasive sampling of saliva outside a clinical setting. Here, we report on the discovery of 35 parasite markers from which we selected a single candidate for use in a PON RDT. We performed a cross-sectional, multi-omics study of saliva from 364 children with subclinical infection in Cameroon and Zambia and produced a prototype saliva-based PON lateral flow immunoassay test for P. falciparum gametocyte carriers. The test is capable of identifying submicroscopic carriage in both clinical and nonclinical settings and is compatible with archived saliva samples.
| Original language | English (US) |
|---|---|
| Article number | eaan4479 |
| Journal | Science Translational Medicine |
| Volume | 11 |
| Issue number | 473 |
| DOIs | |
| State | Published - Jan 2 2019 |
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ASJC Scopus subject areas
- Medicine(all)
Cite this
A saliva-based rapid test to quantify the infectious subclinical malaria parasite reservoir. / Tao, Dingyin; McGill, Brent; Hamerly, Timothy; Kobayashi, Tamaki; Khare, Prachi; Dziedzic, Amanda; Leski, Tomasz; Holtz, Andrew; Shull, Bruce; Jedlicka, Anne Elizabeth; Walzer, Andrew; Slowey, Paul D.; Slowey, Christopher C.; Nsango, Sandrine E.; Stenger, David A.; Chaponda, Mike; Mulenga, Modest; Jacobsen, Kathryn H.; Sullivan, David J; Ryan, Sadie J.; Ansumana, Rashid; Moss, William J; Morlais, Isabelle; Dinglasan, Rhoel R.
In: Science Translational Medicine, Vol. 11, No. 473, eaan4479, 02.01.2019.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A saliva-based rapid test to quantify the infectious subclinical malaria parasite reservoir
AU - Tao, Dingyin
AU - McGill, Brent
AU - Hamerly, Timothy
AU - Kobayashi, Tamaki
AU - Khare, Prachi
AU - Dziedzic, Amanda
AU - Leski, Tomasz
AU - Holtz, Andrew
AU - Shull, Bruce
AU - Jedlicka, Anne Elizabeth
AU - Walzer, Andrew
AU - Slowey, Paul D.
AU - Slowey, Christopher C.
AU - Nsango, Sandrine E.
AU - Stenger, David A.
AU - Chaponda, Mike
AU - Mulenga, Modest
AU - Jacobsen, Kathryn H.
AU - Sullivan, David J
AU - Ryan, Sadie J.
AU - Ansumana, Rashid
AU - Moss, William J
AU - Morlais, Isabelle
AU - Dinglasan, Rhoel R.
PY - 2019/1/2
Y1 - 2019/1/2
N2 - A large proportion of ongoing malaria parasite transmission is attributed to low-density subclinical infections not readily detected by available rapid diagnostic tests (RDTs) or microscopy. Plasmodium falciparum gametocyte carriage is subclinical, but gametocytemic individuals comprise the parasite reservoir that leads to infection of mosquitoes and local transmission. Effective detection and quantification of these carriers can help advance malaria elimination strategies. However, no point-of-need (PON) RDTs for gametocyte detection exist, much less one that can perform noninvasive sampling of saliva outside a clinical setting. Here, we report on the discovery of 35 parasite markers from which we selected a single candidate for use in a PON RDT. We performed a cross-sectional, multi-omics study of saliva from 364 children with subclinical infection in Cameroon and Zambia and produced a prototype saliva-based PON lateral flow immunoassay test for P. falciparum gametocyte carriers. The test is capable of identifying submicroscopic carriage in both clinical and nonclinical settings and is compatible with archived saliva samples.
AB - A large proportion of ongoing malaria parasite transmission is attributed to low-density subclinical infections not readily detected by available rapid diagnostic tests (RDTs) or microscopy. Plasmodium falciparum gametocyte carriage is subclinical, but gametocytemic individuals comprise the parasite reservoir that leads to infection of mosquitoes and local transmission. Effective detection and quantification of these carriers can help advance malaria elimination strategies. However, no point-of-need (PON) RDTs for gametocyte detection exist, much less one that can perform noninvasive sampling of saliva outside a clinical setting. Here, we report on the discovery of 35 parasite markers from which we selected a single candidate for use in a PON RDT. We performed a cross-sectional, multi-omics study of saliva from 364 children with subclinical infection in Cameroon and Zambia and produced a prototype saliva-based PON lateral flow immunoassay test for P. falciparum gametocyte carriers. The test is capable of identifying submicroscopic carriage in both clinical and nonclinical settings and is compatible with archived saliva samples.
UR - http://www.scopus.com/inward/record.url?scp=85059500517&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85059500517&partnerID=8YFLogxK
U2 - 10.1126/scitranslmed.aan4479
DO - 10.1126/scitranslmed.aan4479
M3 - Article
C2 - 30602535
AN - SCOPUS:85059500517
VL - 11
JO - Science Translational Medicine
JF - Science Translational Medicine
SN - 1946-6234
IS - 473
M1 - eaan4479
ER -