A saliva-based rapid test to quantify the infectious subclinical malaria parasite reservoir

Dingyin Tao, Brent McGill, Timothy Hamerly, Tamaki Kobayashi, Prachi Khare, Amanda Dziedzic, Tomasz Leski, Andrew Holtz, Bruce Shull, Anne Elizabeth Jedlicka, Andrew Walzer, Paul D. Slowey, Christopher C. Slowey, Sandrine E. Nsango, David A. Stenger, Mike Chaponda, Modest Mulenga, Kathryn H. Jacobsen, David J Sullivan, Sadie J. RyanRashid Ansumana, William J Moss, Isabelle Morlais, Rhoel R. Dinglasan

Research output: Contribution to journalArticle

Abstract

A large proportion of ongoing malaria parasite transmission is attributed to low-density subclinical infections not readily detected by available rapid diagnostic tests (RDTs) or microscopy. Plasmodium falciparum gametocyte carriage is subclinical, but gametocytemic individuals comprise the parasite reservoir that leads to infection of mosquitoes and local transmission. Effective detection and quantification of these carriers can help advance malaria elimination strategies. However, no point-of-need (PON) RDTs for gametocyte detection exist, much less one that can perform noninvasive sampling of saliva outside a clinical setting. Here, we report on the discovery of 35 parasite markers from which we selected a single candidate for use in a PON RDT. We performed a cross-sectional, multi-omics study of saliva from 364 children with subclinical infection in Cameroon and Zambia and produced a prototype saliva-based PON lateral flow immunoassay test for P. falciparum gametocyte carriers. The test is capable of identifying submicroscopic carriage in both clinical and nonclinical settings and is compatible with archived saliva samples.

Original languageEnglish (US)
Article numbereaan4479
JournalScience Translational Medicine
Volume11
Issue number473
DOIs
StatePublished - Jan 2 2019

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Saliva
Malaria
Parasites
Routine Diagnostic Tests
Asymptomatic Infections
Plasmodium falciparum
Zambia
Cameroon
Culicidae
Immunoassay
Microscopy
Infection

ASJC Scopus subject areas

  • Medicine(all)

Cite this

A saliva-based rapid test to quantify the infectious subclinical malaria parasite reservoir. / Tao, Dingyin; McGill, Brent; Hamerly, Timothy; Kobayashi, Tamaki; Khare, Prachi; Dziedzic, Amanda; Leski, Tomasz; Holtz, Andrew; Shull, Bruce; Jedlicka, Anne Elizabeth; Walzer, Andrew; Slowey, Paul D.; Slowey, Christopher C.; Nsango, Sandrine E.; Stenger, David A.; Chaponda, Mike; Mulenga, Modest; Jacobsen, Kathryn H.; Sullivan, David J; Ryan, Sadie J.; Ansumana, Rashid; Moss, William J; Morlais, Isabelle; Dinglasan, Rhoel R.

In: Science Translational Medicine, Vol. 11, No. 473, eaan4479, 02.01.2019.

Research output: Contribution to journalArticle

Tao, D, McGill, B, Hamerly, T, Kobayashi, T, Khare, P, Dziedzic, A, Leski, T, Holtz, A, Shull, B, Jedlicka, AE, Walzer, A, Slowey, PD, Slowey, CC, Nsango, SE, Stenger, DA, Chaponda, M, Mulenga, M, Jacobsen, KH, Sullivan, DJ, Ryan, SJ, Ansumana, R, Moss, WJ, Morlais, I & Dinglasan, RR 2019, 'A saliva-based rapid test to quantify the infectious subclinical malaria parasite reservoir', Science Translational Medicine, vol. 11, no. 473, eaan4479. https://doi.org/10.1126/scitranslmed.aan4479
Tao, Dingyin ; McGill, Brent ; Hamerly, Timothy ; Kobayashi, Tamaki ; Khare, Prachi ; Dziedzic, Amanda ; Leski, Tomasz ; Holtz, Andrew ; Shull, Bruce ; Jedlicka, Anne Elizabeth ; Walzer, Andrew ; Slowey, Paul D. ; Slowey, Christopher C. ; Nsango, Sandrine E. ; Stenger, David A. ; Chaponda, Mike ; Mulenga, Modest ; Jacobsen, Kathryn H. ; Sullivan, David J ; Ryan, Sadie J. ; Ansumana, Rashid ; Moss, William J ; Morlais, Isabelle ; Dinglasan, Rhoel R. / A saliva-based rapid test to quantify the infectious subclinical malaria parasite reservoir. In: Science Translational Medicine. 2019 ; Vol. 11, No. 473.
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