A role for the 2′ OH of peptidyl-tRNA substrate in peptide release on the ribosome revealed through RF-mediated rescue

Jeffrey J. Shaw, Stefan Trobro, Shan L. He, Johan Qvist, Rachel Green

Research output: Contribution to journalArticle

Abstract

The 2′ OH of the peptidyl-tRNA substrate is thought to be important for catalysis of both peptide bond formation and peptide release in the ribosomal active site. The release reaction also specifically depends on a release factor protein (RF) to hydrolyze the ester linkage of the peptidyl-tRNA upon recognition of stop codons in the A site. Here, we demonstrate that certain amino acid substitutions (in particular those containing hydroxyl or thiol groups) in the conserved GGQ glutamine of release factor RF1 can rescue defects in the release reaction associated with peptidyl-tRNA substrates lacking a 2′ OH. We explored this rescue effect through biochemical and computational approaches that support a model where the 2′ OH of the P-site substrate is critical for orienting the nucleophile in a hydrogen-bonding network productive for catalysis.

Original languageEnglish (US)
Pages (from-to)983-993
Number of pages11
JournalChemistry and Biology
Volume19
Issue number8
DOIs
StatePublished - Aug 24 2012

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmacology
  • Drug Discovery
  • Clinical Biochemistry

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