A role for heparan sulfate proteoglycans in Plasmodium falciparum sporozoite invasion of anopheline mosquito salivary glands

Jennifer S. Armistead, Iain B H Wilson, Toin H. Van Kuppevelt, Rhoel R. Dinglasan

Research output: Contribution to journalArticle

Abstract

HS (heparan sulfate) has been shown to be an important mediator of Plasmodium sporozoite homing and invasion of the liver, but the role of this glycosaminoglycan in mosquito vector host-sporozoite interactions is unknown. We have biochemically characterized the function of AgOXT1 (Anopheles gambiae peptide-O-xylosyltransferase 1) and confirmed that AgOXT1 can modify peptides representing model HS and chondroitin sulfate proteoglycans in vitro. Moreover, we also demonstrated that the mosquito salivary gland basal lamina proteoglycans are modified by HS. We used RNA interference-mediated knockdown of HS biosynthesis in A. gambiae salivary glands to determine whether Plasmodium falciparum sporozoites that are released frommosquito midgut oocysts use salivary gland HS as a receptor for tissue invasion. Our results suggest that salivary gland basal lamina HS glycosaminoglycans only partially mediate midgut sporozoite invasion of this tissue, and that in the absence of HS, the presence of other surface co-receptors is sufficient to facilitate parasite entry.

Original languageEnglish (US)
Pages (from-to)475-483
Number of pages9
JournalBiochemical Journal
Volume438
Issue number3
DOIs
StatePublished - Sep 15 2011

Fingerprint

Sporozoites
Heparan Sulfate Proteoglycans
Heparitin Sulfate
Plasmodium falciparum
Salivary Glands
Culicidae
Anopheles gambiae
Glycosaminoglycans
Basement Membrane
Peptides
Chondroitin Sulfate Proteoglycans
Tissue
Plasmodium
Oocysts
RNA Interference
Biosynthesis
Proteoglycans
Parasites
Liver
RNA

Keywords

  • Anopheles
  • Cell invasion
  • Glycobiology
  • Glycosaminoglycan (GAG)
  • Heparan sulfate (HS)
  • Malaria

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

A role for heparan sulfate proteoglycans in Plasmodium falciparum sporozoite invasion of anopheline mosquito salivary glands. / Armistead, Jennifer S.; Wilson, Iain B H; Van Kuppevelt, Toin H.; Dinglasan, Rhoel R.

In: Biochemical Journal, Vol. 438, No. 3, 15.09.2011, p. 475-483.

Research output: Contribution to journalArticle

Armistead, Jennifer S. ; Wilson, Iain B H ; Van Kuppevelt, Toin H. ; Dinglasan, Rhoel R. / A role for heparan sulfate proteoglycans in Plasmodium falciparum sporozoite invasion of anopheline mosquito salivary glands. In: Biochemical Journal. 2011 ; Vol. 438, No. 3. pp. 475-483.
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