A reversed-phase high-performance liquid chromatographic method for the determination of Clanfenur in rat and human plasma

H. P J M Noteborn; F. J. Varossieau; M. Monshouwer; R. Brands; H. M. Pinedo

doi:10.1016/0731-7085(94)E0013-Q

A reversed-phase high-performance liquid chromatographic method for the determination of Clanfenur in rat and human plasma

H. P J M Noteborn, F. J. Varossieau, M. Monshouwer, R. Brands, H. M. Pinedo

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

A selective and specific high-pressure liquid chromatographic (HPLC) method for the simultaneous assay of Clanfenur and its metabolites in biological fluids of interest has been developed which is suitable for routine analysis, using micro volumes (0.1 ml) of plasma samples only. After protein precipitation the extract is analysed by reversed-phase HPLC with UV detection. Excellent recovery, linearity, accuracy and precision (less than 5% for plasma) are achieved by the assay which is able to quantify Clanfenur and its metabolises in plasma at concentrations between 0.025 and 5.0 mg l^-1.

Original language	English (US)
Pages (from-to)	937-942
Number of pages	6
Journal	Journal of Pharmaceutical and Biomedical Analysis
Volume	12
Issue number	7
DOIs	https://doi.org/10.1016/0731-7085(94)E0013-Q
State	Published - 1994
Externally published	Yes

Keywords

antineoplastic drugs.
benzoylphenyl ureas
Clanfenur
reversed-phase HPLC

ASJC Scopus subject areas

Analytical Chemistry
Pharmaceutical Science

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10.1016/0731-7085(94)E0013-Q

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title = "A reversed-phase high-performance liquid chromatographic method for the determination of Clanfenur in rat and human plasma",

abstract = "A selective and specific high-pressure liquid chromatographic (HPLC) method for the simultaneous assay of Clanfenur and its metabolites in biological fluids of interest has been developed which is suitable for routine analysis, using micro volumes (0.1 ml) of plasma samples only. After protein precipitation the extract is analysed by reversed-phase HPLC with UV detection. Excellent recovery, linearity, accuracy and precision (less than 5% for plasma) are achieved by the assay which is able to quantify Clanfenur and its metabolises in plasma at concentrations between 0.025 and 5.0 mg l-1.",

keywords = "antineoplastic drugs., benzoylphenyl ureas, Clanfenur, reversed-phase HPLC",

author = "Noteborn, {H. P J M} and Varossieau, {F. J.} and M. Monshouwer and R. Brands and Pinedo, {H. M.}",

year = "1994",

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T1 - A reversed-phase high-performance liquid chromatographic method for the determination of Clanfenur in rat and human plasma

AU - Noteborn, H. P J M

AU - Varossieau, F. J.

AU - Monshouwer, M.

AU - Brands, R.

AU - Pinedo, H. M.

PY - 1994

Y1 - 1994

N2 - A selective and specific high-pressure liquid chromatographic (HPLC) method for the simultaneous assay of Clanfenur and its metabolites in biological fluids of interest has been developed which is suitable for routine analysis, using micro volumes (0.1 ml) of plasma samples only. After protein precipitation the extract is analysed by reversed-phase HPLC with UV detection. Excellent recovery, linearity, accuracy and precision (less than 5% for plasma) are achieved by the assay which is able to quantify Clanfenur and its metabolises in plasma at concentrations between 0.025 and 5.0 mg l-1.

AB - A selective and specific high-pressure liquid chromatographic (HPLC) method for the simultaneous assay of Clanfenur and its metabolites in biological fluids of interest has been developed which is suitable for routine analysis, using micro volumes (0.1 ml) of plasma samples only. After protein precipitation the extract is analysed by reversed-phase HPLC with UV detection. Excellent recovery, linearity, accuracy and precision (less than 5% for plasma) are achieved by the assay which is able to quantify Clanfenur and its metabolises in plasma at concentrations between 0.025 and 5.0 mg l-1.

KW - antineoplastic drugs.

KW - benzoylphenyl ureas

KW - Clanfenur

KW - reversed-phase HPLC

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A reversed-phase high-performance liquid chromatographic method for the determination of Clanfenur in rat and human plasma

Abstract

Keywords

ASJC Scopus subject areas

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