A reversed-phase high-performance liquid chromatographic method for the determination of Clanfenur in rat and human plasma

H. P J M Noteborn, F. J. Varossieau, M. Monshouwer, R. Brands, H. M. Pinedo

Research output: Contribution to journalArticlepeer-review

Abstract

A selective and specific high-pressure liquid chromatographic (HPLC) method for the simultaneous assay of Clanfenur and its metabolites in biological fluids of interest has been developed which is suitable for routine analysis, using micro volumes (0.1 ml) of plasma samples only. After protein precipitation the extract is analysed by reversed-phase HPLC with UV detection. Excellent recovery, linearity, accuracy and precision (less than 5% for plasma) are achieved by the assay which is able to quantify Clanfenur and its metabolises in plasma at concentrations between 0.025 and 5.0 mg l-1.

Original languageEnglish (US)
Pages (from-to)937-942
Number of pages6
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume12
Issue number7
DOIs
StatePublished - 1994
Externally publishedYes

Keywords

  • antineoplastic drugs.
  • benzoylphenyl ureas
  • Clanfenur
  • reversed-phase HPLC

ASJC Scopus subject areas

  • Analytical Chemistry
  • Pharmaceutical Science

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