A retrospective analysis of false-positive infectious screening results in blood donors

Michelle T. Vo, Roberta Bruhn, Zhanna Kaidarova, Brian S. Custer, Edward L. Murphy, Evan Bloch

Research output: Contribution to journalArticle

Abstract

BACKGROUND False-positive infectious transfusion screening results remain a challenge with continued loss of both donors and blood products. We sought to identify associations between donor demographic characteristics (age, race, sex, education, first-time donor status) and testing false positive for viruses during routine blood donation screening. In addition the study assessed the prevalence of high-risk behaviors in false-positive donors. STUDY DESIGN AND METHODS Blood Systems, Inc. donors with allogeneic donations between January 1, 2011, and December 31, 2012, were compared in a case-control study. Those with a false-positive donation for one of four viruses (human immunodeficiency virus [HIV], human T-lymphotropic virus [HTLV], hepatitis B virus [HBV], and hepatitis C virus [HCV]) were included as cases. Those with negative test results were controls. For a subset of cases, infectious risk factors were evaluated. RESULTS Black race and Hispanic ethnicity were associated with HCV and HTLV false-positive results. Male sex and lower education were associated with HCV false positivity, and age 25 to 44 was associated with HTLV false positivity. First-time donors were more likely to be HCV false positive although less likely to be HBV and HTLV false positive. No significant associations between donor demographics and HIV false positivity were observed. A questionnaire for false-positive donors showed low levels of high-risk behaviors. CONCLUSION Demographic associations with HCV and HTLV false-positive results overlap with those of true infection. While true infection is unlikely given current testing algorithms and risk factor evaluation, the findings suggest nonrandom association. Further investigation into biologic mechanisms is warranted.

Original languageEnglish (US)
Pages (from-to)457-465
Number of pages9
JournalTransfusion
Volume56
Issue number2
DOIs
StatePublished - Feb 1 2016
Externally publishedYes

Fingerprint

Blood Donors
Tissue Donors
Hepacivirus
Viruses
Sex Education
Demography
Risk-Taking
Hepatitis B virus
HIV
Infection
Hispanic Americans
Case-Control Studies
Cross-Sectional Studies

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Hematology

Cite this

A retrospective analysis of false-positive infectious screening results in blood donors. / Vo, Michelle T.; Bruhn, Roberta; Kaidarova, Zhanna; Custer, Brian S.; Murphy, Edward L.; Bloch, Evan.

In: Transfusion, Vol. 56, No. 2, 01.02.2016, p. 457-465.

Research output: Contribution to journalArticle

Vo, MT, Bruhn, R, Kaidarova, Z, Custer, BS, Murphy, EL & Bloch, E 2016, 'A retrospective analysis of false-positive infectious screening results in blood donors', Transfusion, vol. 56, no. 2, pp. 457-465. https://doi.org/10.1111/trf.13381
Vo, Michelle T. ; Bruhn, Roberta ; Kaidarova, Zhanna ; Custer, Brian S. ; Murphy, Edward L. ; Bloch, Evan. / A retrospective analysis of false-positive infectious screening results in blood donors. In: Transfusion. 2016 ; Vol. 56, No. 2. pp. 457-465.
@article{4b4f6a465f0c4e03afcdcf194d5541e1,
title = "A retrospective analysis of false-positive infectious screening results in blood donors",
abstract = "BACKGROUND False-positive infectious transfusion screening results remain a challenge with continued loss of both donors and blood products. We sought to identify associations between donor demographic characteristics (age, race, sex, education, first-time donor status) and testing false positive for viruses during routine blood donation screening. In addition the study assessed the prevalence of high-risk behaviors in false-positive donors. STUDY DESIGN AND METHODS Blood Systems, Inc. donors with allogeneic donations between January 1, 2011, and December 31, 2012, were compared in a case-control study. Those with a false-positive donation for one of four viruses (human immunodeficiency virus [HIV], human T-lymphotropic virus [HTLV], hepatitis B virus [HBV], and hepatitis C virus [HCV]) were included as cases. Those with negative test results were controls. For a subset of cases, infectious risk factors were evaluated. RESULTS Black race and Hispanic ethnicity were associated with HCV and HTLV false-positive results. Male sex and lower education were associated with HCV false positivity, and age 25 to 44 was associated with HTLV false positivity. First-time donors were more likely to be HCV false positive although less likely to be HBV and HTLV false positive. No significant associations between donor demographics and HIV false positivity were observed. A questionnaire for false-positive donors showed low levels of high-risk behaviors. CONCLUSION Demographic associations with HCV and HTLV false-positive results overlap with those of true infection. While true infection is unlikely given current testing algorithms and risk factor evaluation, the findings suggest nonrandom association. Further investigation into biologic mechanisms is warranted.",
author = "Vo, {Michelle T.} and Roberta Bruhn and Zhanna Kaidarova and Custer, {Brian S.} and Murphy, {Edward L.} and Evan Bloch",
year = "2016",
month = "2",
day = "1",
doi = "10.1111/trf.13381",
language = "English (US)",
volume = "56",
pages = "457--465",
journal = "Transfusion",
issn = "0041-1132",
publisher = "Wiley-Blackwell",
number = "2",

}

TY - JOUR

T1 - A retrospective analysis of false-positive infectious screening results in blood donors

AU - Vo, Michelle T.

AU - Bruhn, Roberta

AU - Kaidarova, Zhanna

AU - Custer, Brian S.

AU - Murphy, Edward L.

AU - Bloch, Evan

PY - 2016/2/1

Y1 - 2016/2/1

N2 - BACKGROUND False-positive infectious transfusion screening results remain a challenge with continued loss of both donors and blood products. We sought to identify associations between donor demographic characteristics (age, race, sex, education, first-time donor status) and testing false positive for viruses during routine blood donation screening. In addition the study assessed the prevalence of high-risk behaviors in false-positive donors. STUDY DESIGN AND METHODS Blood Systems, Inc. donors with allogeneic donations between January 1, 2011, and December 31, 2012, were compared in a case-control study. Those with a false-positive donation for one of four viruses (human immunodeficiency virus [HIV], human T-lymphotropic virus [HTLV], hepatitis B virus [HBV], and hepatitis C virus [HCV]) were included as cases. Those with negative test results were controls. For a subset of cases, infectious risk factors were evaluated. RESULTS Black race and Hispanic ethnicity were associated with HCV and HTLV false-positive results. Male sex and lower education were associated with HCV false positivity, and age 25 to 44 was associated with HTLV false positivity. First-time donors were more likely to be HCV false positive although less likely to be HBV and HTLV false positive. No significant associations between donor demographics and HIV false positivity were observed. A questionnaire for false-positive donors showed low levels of high-risk behaviors. CONCLUSION Demographic associations with HCV and HTLV false-positive results overlap with those of true infection. While true infection is unlikely given current testing algorithms and risk factor evaluation, the findings suggest nonrandom association. Further investigation into biologic mechanisms is warranted.

AB - BACKGROUND False-positive infectious transfusion screening results remain a challenge with continued loss of both donors and blood products. We sought to identify associations between donor demographic characteristics (age, race, sex, education, first-time donor status) and testing false positive for viruses during routine blood donation screening. In addition the study assessed the prevalence of high-risk behaviors in false-positive donors. STUDY DESIGN AND METHODS Blood Systems, Inc. donors with allogeneic donations between January 1, 2011, and December 31, 2012, were compared in a case-control study. Those with a false-positive donation for one of four viruses (human immunodeficiency virus [HIV], human T-lymphotropic virus [HTLV], hepatitis B virus [HBV], and hepatitis C virus [HCV]) were included as cases. Those with negative test results were controls. For a subset of cases, infectious risk factors were evaluated. RESULTS Black race and Hispanic ethnicity were associated with HCV and HTLV false-positive results. Male sex and lower education were associated with HCV false positivity, and age 25 to 44 was associated with HTLV false positivity. First-time donors were more likely to be HCV false positive although less likely to be HBV and HTLV false positive. No significant associations between donor demographics and HIV false positivity were observed. A questionnaire for false-positive donors showed low levels of high-risk behaviors. CONCLUSION Demographic associations with HCV and HTLV false-positive results overlap with those of true infection. While true infection is unlikely given current testing algorithms and risk factor evaluation, the findings suggest nonrandom association. Further investigation into biologic mechanisms is warranted.

UR - http://www.scopus.com/inward/record.url?scp=84958856048&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84958856048&partnerID=8YFLogxK

U2 - 10.1111/trf.13381

DO - 10.1111/trf.13381

M3 - Article

VL - 56

SP - 457

EP - 465

JO - Transfusion

JF - Transfusion

SN - 0041-1132

IS - 2

ER -