Abstract
When Atlantic salmon (Salmo salar) genomic DNA is digested with the restriction endonuclease BglI and the fragments separated by agarose-gel electrophoresis, bands corresponding to approximately 430 and 923 bp are visualized after EtdBr staining. The 923-bp band was excised from a preparative gel and used to screen a salmon genomic library for recombinant phage (re-phage) containing the repeat. The BglI repeat element is tandemly arrayed, and an array from one re-phage has been sequenced. The BglI repeats comprise 2.3% of the S. salar genome and have been found in the vicinity of rDNA genes (encoding ribosomal RNA). Southern blot hybridization detects a homologue of the Atlantic salmon BglI repeat in the brown trout (Salmo trutta) genome, but not in other salmonids. However, a DNA fragment with sequence homology to part of the BglI repeat has recently been isolated from Arctic charr (Salvelinus alpinus; S.E. Hartley and W.S.D., unpublished data). In addition, the BglI repeat detects RFLPs in Atlantic salmon.
Original language | English (US) |
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Pages (from-to) | 237-242 |
Number of pages | 6 |
Journal | Gene |
Volume | 131 |
Issue number | 2 |
DOIs | |
State | Published - Sep 15 1993 |
Externally published | Yes |
Keywords
- Genome structure
- RFLP
- recombinant DNA
- ribosomal RNA
- salmonid
- tandem repeats
ASJC Scopus subject areas
- Genetics