A reliable and inexpensive EasyCD4 assay for monitoring HIV-infected individuals in resource-limited settings

Pachamuthu Balakrishnan; Suniti Solomon; Janardhanan Mohanakrishnan; Anitha J. Cecelia; Nagalingeswaran Kumarasamy; Kailapuri G. Murugavel; Bhavani Venkatakrishnan; Sunil S. Solomon; Suzanne M. Crowe; Aylur K. Ganesh; Sadras P. Thyagarajan; Timothy Flanigan; Kenneth H. Mayer

doi:10.1097/01.qai.0000227791.92203.d5

A reliable and inexpensive EasyCD4 assay for monitoring HIV-infected individuals in resource-limited settings

Pachamuthu Balakrishnan, Suniti Solomon, Janardhanan Mohanakrishnan, Anitha J. Cecelia, Nagalingeswaran Kumarasamy, Kailapuri G. Murugavel, Bhavani Venkatakrishnan, Sunil S. Solomon, Suzanne M. Crowe, Aylur K. Ganesh, Sadras P. Thyagarajan, Timothy Flanigan, Kenneth H. Mayer

Research output: Contribution to journal › Article › peer-review

Abstract

Serial measurements of absolute CD4 T-lymphocyte counts are required to initiate and gauge response to therapy and monitor disease progression. Hence, there is an urgent need to evaluate the accuracy and validity of low-cost CD4 T-cell count assays. Tripotassium EDTA blood specimens from HIV-infected individuals were studied using a novel flow cytometric assay (EasyCD4 assay; Guava Technologies, Hayward, CA) in comparison with standard flow cytometry (FACSCount; Becton Dickinson Immunocytometry Systems, San Jose, CA). The sensitivity, specificity value by EasyCD4 assay in enumerating absolute CD4 T-cell counts of less than 200 cells/μL were 95% and 100%, respectively. Bland-Altman analysis showed close agreement, with the EasyCD4 assay yielding CD4 T-cell counts a mean difference of -26 cells/μL (95% confidence interval, -96 to 44 cells/μL) higher than by flow cytometry. Our data suggest that EasyCD4 assay could be a useful alternative assay to conventional flow cytometry, may be appropriate for use in resource-limited settings.

Original language	English (US)
Pages (from-to)	23-26
Number of pages	4
Journal	Journal of acquired immune deficiency syndromes
Volume	43
Issue number	1
DOIs	https://doi.org/10.1097/01.qai.0000227791.92203.d5
State	Published - Sep 2006
Externally published	Yes

Keywords

CD4 T-lymphocyte count
EasyCD4 assay
Flow cytometry
Guava technologies
HIV monitoring
Low-cost CD4
Resource-limited setting

ASJC Scopus subject areas

Infectious Diseases
Pharmacology (medical)

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Balakrishnan, P., Solomon, S., Mohanakrishnan, J., Cecelia, A. J., Kumarasamy, N., Murugavel, K. G., Venkatakrishnan, B., Solomon, S. S., Crowe, S. M., Ganesh, A. K., Thyagarajan, S. P., Flanigan, T., & Mayer, K. H. (2006). A reliable and inexpensive EasyCD4 assay for monitoring HIV-infected individuals in resource-limited settings. Journal of acquired immune deficiency syndromes, 43(1), 23-26. https://doi.org/10.1097/01.qai.0000227791.92203.d5

A reliable and inexpensive EasyCD4 assay for monitoring HIV-infected individuals in resource-limited settings. / Balakrishnan, Pachamuthu; Solomon, Suniti; Mohanakrishnan, Janardhanan; Cecelia, Anitha J.; Kumarasamy, Nagalingeswaran; Murugavel, Kailapuri G.; Venkatakrishnan, Bhavani; Solomon, Sunil S.; Crowe, Suzanne M.; Ganesh, Aylur K.; Thyagarajan, Sadras P.; Flanigan, Timothy; Mayer, Kenneth H.

In: Journal of acquired immune deficiency syndromes, Vol. 43, No. 1, 09.2006, p. 23-26.

Research output: Contribution to journal › Article › peer-review

Balakrishnan, P, Solomon, S, Mohanakrishnan, J, Cecelia, AJ, Kumarasamy, N, Murugavel, KG, Venkatakrishnan, B, Solomon, SS, Crowe, SM, Ganesh, AK, Thyagarajan, SP, Flanigan, T & Mayer, KH 2006, 'A reliable and inexpensive EasyCD4 assay for monitoring HIV-infected individuals in resource-limited settings', Journal of acquired immune deficiency syndromes, vol. 43, no. 1, pp. 23-26. https://doi.org/10.1097/01.qai.0000227791.92203.d5

Balakrishnan, Pachamuthu ; Solomon, Suniti ; Mohanakrishnan, Janardhanan ; Cecelia, Anitha J. ; Kumarasamy, Nagalingeswaran ; Murugavel, Kailapuri G. ; Venkatakrishnan, Bhavani ; Solomon, Sunil S. ; Crowe, Suzanne M. ; Ganesh, Aylur K. ; Thyagarajan, Sadras P. ; Flanigan, Timothy ; Mayer, Kenneth H. / A reliable and inexpensive EasyCD4 assay for monitoring HIV-infected individuals in resource-limited settings. In: Journal of acquired immune deficiency syndromes. 2006 ; Vol. 43, No. 1. pp. 23-26.

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abstract = "Serial measurements of absolute CD4 T-lymphocyte counts are required to initiate and gauge response to therapy and monitor disease progression. Hence, there is an urgent need to evaluate the accuracy and validity of low-cost CD4 T-cell count assays. Tripotassium EDTA blood specimens from HIV-infected individuals were studied using a novel flow cytometric assay (EasyCD4 assay; Guava Technologies, Hayward, CA) in comparison with standard flow cytometry (FACSCount; Becton Dickinson Immunocytometry Systems, San Jose, CA). The sensitivity, specificity value by EasyCD4 assay in enumerating absolute CD4 T-cell counts of less than 200 cells/μL were 95% and 100%, respectively. Bland-Altman analysis showed close agreement, with the EasyCD4 assay yielding CD4 T-cell counts a mean difference of -26 cells/μL (95% confidence interval, -96 to 44 cells/μL) higher than by flow cytometry. Our data suggest that EasyCD4 assay could be a useful alternative assay to conventional flow cytometry, may be appropriate for use in resource-limited settings.",

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A reliable and inexpensive EasyCD4 assay for monitoring HIV-infected individuals in resource-limited settings

Abstract

Keywords

ASJC Scopus subject areas

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