A red-shifted fluorescent substrate for aldehyde dehydrogenase

Il Minn, Haofan Wang, Ronnie C. Mease, Youngjoo Byun, Xing Yang, Julia Wang, Steven D. Leach, Martin G. Pomper

Research output: Contribution to journalArticle

Abstract

Selection of cells positive for aldehyde dehydrogenase (ALDH) activity from a green-fluorescent background is difficult with existing reagents. Here we report a red-shifted fluorescent substrate for ALDH, AldeRed 588-A, for labelling viable ALDH pos cells. We demonstrate that AldeRed 588-A successfully isolates ALDH hi human haematopoietic stem cells from heterogeneous cord blood mononuclear cells. AldeRed 588-A can be used for multicolour applications to fractionate ALDH pos cells in the presence of green fluorophores including the ALDEFLUOR reagent and cells expressing enhanced green-fluorescent protein (eGFP). AldeRed 588-A stains ALDH pos murine pancreatic centroacinar and terminal duct cells, as visualized using fluorescent microscopy. AldeRed588-A provides a useful tool to select stem cells or study ALDH within a green-fluorescent background.

Original languageEnglish (US)
Article number3662
JournalNature communications
Volume5
DOIs
StatePublished - Apr 23 2014

    Fingerprint

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this