A red-shifted fluorescent substrate for aldehyde dehydrogenase

Il Minn; Haofan Wang; Ronnie C. Mease; Youngjoo Byun; Xing Yang; Julia Wang; Steven D. Leach; Martin G. Pomper

doi:10.1038/ncomms4662

A red-shifted fluorescent substrate for aldehyde dehydrogenase

Il Minn, Haofan Wang, Ronnie C. Mease, Youngjoo Byun, Xing Yang, Julia Wang, Steven D. Leach, Martin G. Pomper

School of Medicine

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Selection of cells positive for aldehyde dehydrogenase (ALDH) activity from a green-fluorescent background is difficult with existing reagents. Here we report a red-shifted fluorescent substrate for ALDH, AldeRed 588-A, for labelling viable ALDH pos cells. We demonstrate that AldeRed 588-A successfully isolates ALDH hi human haematopoietic stem cells from heterogeneous cord blood mononuclear cells. AldeRed 588-A can be used for multicolour applications to fractionate ALDH pos cells in the presence of green fluorophores including the ALDEFLUOR reagent and cells expressing enhanced green-fluorescent protein (eGFP). AldeRed 588-A stains ALDH pos murine pancreatic centroacinar and terminal duct cells, as visualized using fluorescent microscopy. AldeRed588-A provides a useful tool to select stem cells or study ALDH within a green-fluorescent background.

Original language	English (US)
Article number	3662
Journal	Nature communications
Volume	5
DOIs	https://doi.org/10.1038/ncomms4662
State	Published - Apr 23 2014

ASJC Scopus subject areas

General Chemistry
General Biochemistry, Genetics and Molecular Biology
General Physics and Astronomy

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10.1038/ncomms4662

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title = "A red-shifted fluorescent substrate for aldehyde dehydrogenase",

abstract = "Selection of cells positive for aldehyde dehydrogenase (ALDH) activity from a green-fluorescent background is difficult with existing reagents. Here we report a red-shifted fluorescent substrate for ALDH, AldeRed 588-A, for labelling viable ALDH pos cells. We demonstrate that AldeRed 588-A successfully isolates ALDH hi human haematopoietic stem cells from heterogeneous cord blood mononuclear cells. AldeRed 588-A can be used for multicolour applications to fractionate ALDH pos cells in the presence of green fluorophores including the ALDEFLUOR reagent and cells expressing enhanced green-fluorescent protein (eGFP). AldeRed 588-A stains ALDH pos murine pancreatic centroacinar and terminal duct cells, as visualized using fluorescent microscopy. AldeRed588-A provides a useful tool to select stem cells or study ALDH within a green-fluorescent background.",

author = "Il Minn and Haofan Wang and Mease, {Ronnie C.} and Youngjoo Byun and Xing Yang and Julia Wang and Leach, {Steven D.} and Pomper, {Martin G.}",

note = "Funding Information: We would like to thank Dr Crystal Dusich for the synthesis of 1. We also thank (all from Johns Hopkins) Drs Shyam Biswal and Jung-Hyun Kim for providing the TNR mice, Dr Richard Jones for providing L1210/cpa and L1210 cell lines, Dr Wojciech Lesniak for helping with excitation and emission profiling and Dr Hao Zhang and Ada Tam for providing guidance with FACS analyses. This work was funded by Maryland Stem Cell Research Fund grants 2010-MSCRFF-0109-00 and 2012-MSCRFE-0211-00 and NIH DK56211, CA134675 and CA098252.",

year = "2014",

month = apr,

day = "23",

doi = "10.1038/ncomms4662",

language = "English (US)",

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AU - Minn, Il

AU - Wang, Haofan

AU - Mease, Ronnie C.

AU - Byun, Youngjoo

AU - Yang, Xing

AU - Wang, Julia

AU - Leach, Steven D.

AU - Pomper, Martin G.

N1 - Funding Information: We would like to thank Dr Crystal Dusich for the synthesis of 1. We also thank (all from Johns Hopkins) Drs Shyam Biswal and Jung-Hyun Kim for providing the TNR mice, Dr Richard Jones for providing L1210/cpa and L1210 cell lines, Dr Wojciech Lesniak for helping with excitation and emission profiling and Dr Hao Zhang and Ada Tam for providing guidance with FACS analyses. This work was funded by Maryland Stem Cell Research Fund grants 2010-MSCRFF-0109-00 and 2012-MSCRFE-0211-00 and NIH DK56211, CA134675 and CA098252.

PY - 2014/4/23

Y1 - 2014/4/23

N2 - Selection of cells positive for aldehyde dehydrogenase (ALDH) activity from a green-fluorescent background is difficult with existing reagents. Here we report a red-shifted fluorescent substrate for ALDH, AldeRed 588-A, for labelling viable ALDH pos cells. We demonstrate that AldeRed 588-A successfully isolates ALDH hi human haematopoietic stem cells from heterogeneous cord blood mononuclear cells. AldeRed 588-A can be used for multicolour applications to fractionate ALDH pos cells in the presence of green fluorophores including the ALDEFLUOR reagent and cells expressing enhanced green-fluorescent protein (eGFP). AldeRed 588-A stains ALDH pos murine pancreatic centroacinar and terminal duct cells, as visualized using fluorescent microscopy. AldeRed588-A provides a useful tool to select stem cells or study ALDH within a green-fluorescent background.

AB - Selection of cells positive for aldehyde dehydrogenase (ALDH) activity from a green-fluorescent background is difficult with existing reagents. Here we report a red-shifted fluorescent substrate for ALDH, AldeRed 588-A, for labelling viable ALDH pos cells. We demonstrate that AldeRed 588-A successfully isolates ALDH hi human haematopoietic stem cells from heterogeneous cord blood mononuclear cells. AldeRed 588-A can be used for multicolour applications to fractionate ALDH pos cells in the presence of green fluorophores including the ALDEFLUOR reagent and cells expressing enhanced green-fluorescent protein (eGFP). AldeRed 588-A stains ALDH pos murine pancreatic centroacinar and terminal duct cells, as visualized using fluorescent microscopy. AldeRed588-A provides a useful tool to select stem cells or study ALDH within a green-fluorescent background.

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A red-shifted fluorescent substrate for aldehyde dehydrogenase

Abstract

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