A red-shifted fluorescent substrate for aldehyde dehydrogenase

Il Minn, Haofan Wang, Ronnie Mease, Youngjoo Byun, Xing Yang, Julia Wang, Steven D. Leach, Martin Gilbert Pomper

Research output: Contribution to journalArticle

Abstract

Selection of cells positive for aldehyde dehydrogenase (ALDH) activity from a green-fluorescent background is difficult with existing reagents. Here we report a red-shifted fluorescent substrate for ALDH, AldeRed 588-A, for labelling viable ALDH pos cells. We demonstrate that AldeRed 588-A successfully isolates ALDH hi human haematopoietic stem cells from heterogeneous cord blood mononuclear cells. AldeRed 588-A can be used for multicolour applications to fractionate ALDH pos cells in the presence of green fluorophores including the ALDEFLUOR reagent and cells expressing enhanced green-fluorescent protein (eGFP). AldeRed 588-A stains ALDH pos murine pancreatic centroacinar and terminal duct cells, as visualized using fluorescent microscopy. AldeRed588-A provides a useful tool to select stem cells or study ALDH within a green-fluorescent background.

Original languageEnglish (US)
Article number3662
JournalNature Communications
Volume5
DOIs
StatePublished - Apr 23 2014

Fingerprint

Aldehyde Dehydrogenase
dehydrogenases
aldehydes
Substrates
stem cells
Stem cells
cells
reagents
blood cells
Fluorophores
Hematopoietic Stem Cells
Fetal Blood
ducts
Ducts
Labeling
marking
Microscopy
Blood Cells
Microscopic examination
Blood

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry(all)
  • Physics and Astronomy(all)
  • Medicine(all)

Cite this

A red-shifted fluorescent substrate for aldehyde dehydrogenase. / Minn, Il; Wang, Haofan; Mease, Ronnie; Byun, Youngjoo; Yang, Xing; Wang, Julia; Leach, Steven D.; Pomper, Martin Gilbert.

In: Nature Communications, Vol. 5, 3662, 23.04.2014.

Research output: Contribution to journalArticle

Minn, Il ; Wang, Haofan ; Mease, Ronnie ; Byun, Youngjoo ; Yang, Xing ; Wang, Julia ; Leach, Steven D. ; Pomper, Martin Gilbert. / A red-shifted fluorescent substrate for aldehyde dehydrogenase. In: Nature Communications. 2014 ; Vol. 5.
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