A recurrent gain of chromosome arm 3q in primary squamous carcinoma of the vagina

Jens K. Habermann, Kristina Hellman, Sandra Freitag, Kerstin Heselmeyer-Haddad, Ann Cathrin Hellström, Keerti Shah, Gert Auer, Thomas Ried

Research output: Contribution to journalArticle

Abstract

Primary carcinomas of the vagina are rare tumors, accounting for 2%-3% of all gynecologic malignancies. Only a few karyotypes based on chromosome banding techniques have been reported. We have, therefore, used comparative genomic hybridization to establish a pattern of genomic imbalances in vaginal squamous cell carcinomas. Analysis of 16 formalin-fixed and paraffin-embedded tumors revealed that 70% of vaginal carcinomas carry relative copy number increases that map to chromosome arm 3q. Other recurring gains were observed on chromosome arms 5p and 19p. Chromosomal losses were infrequent. Most tumors were aneuploid, as measured by image cytometry on Feulgen-stained tissue sections. The cytogenetic data were related to the presence of human papillomavirus genomes, expression of laminin-5 as a marker for invasiveness, and expression levels of markers for proliferative activity and mutated TP53. All relevant clinical data were recorded. The results suggest that vaginal carcinomas are defined by a specific distribution of chromosomal aneuploidies and that the pattern of genomic imbalances is strikingly similar to that observed in squamous cell carcinomas of the uterine cervix. Age at diagnosis (P=0.031), tumor size (P=0.025), and increased laminin-5 expression (P=0.006) have a significant influence on the survival time.

Original languageEnglish (US)
Pages (from-to)7-13
Number of pages7
JournalCancer Genetics and Cytogenetics
Volume148
Issue number1
DOIs
StatePublished - Jan 1 2004

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Vagina
Squamous Cell Carcinoma
Chromosomes
Aneuploidy
Neoplasms
Carcinoma
Image Cytometry
Chromosome Banding
Comparative Genomic Hybridization
Human Genome
Karyotype
Cervix Uteri
Cytogenetics
Paraffin
Formaldehyde
kalinin

ASJC Scopus subject areas

  • Cancer Research
  • Genetics
  • Molecular Biology

Cite this

Habermann, J. K., Hellman, K., Freitag, S., Heselmeyer-Haddad, K., Hellström, A. C., Shah, K., ... Ried, T. (2004). A recurrent gain of chromosome arm 3q in primary squamous carcinoma of the vagina. Cancer Genetics and Cytogenetics, 148(1), 7-13. https://doi.org/10.1016/S0165-4608(03)00245-0

A recurrent gain of chromosome arm 3q in primary squamous carcinoma of the vagina. / Habermann, Jens K.; Hellman, Kristina; Freitag, Sandra; Heselmeyer-Haddad, Kerstin; Hellström, Ann Cathrin; Shah, Keerti; Auer, Gert; Ried, Thomas.

In: Cancer Genetics and Cytogenetics, Vol. 148, No. 1, 01.01.2004, p. 7-13.

Research output: Contribution to journalArticle

Habermann, JK, Hellman, K, Freitag, S, Heselmeyer-Haddad, K, Hellström, AC, Shah, K, Auer, G & Ried, T 2004, 'A recurrent gain of chromosome arm 3q in primary squamous carcinoma of the vagina', Cancer Genetics and Cytogenetics, vol. 148, no. 1, pp. 7-13. https://doi.org/10.1016/S0165-4608(03)00245-0
Habermann JK, Hellman K, Freitag S, Heselmeyer-Haddad K, Hellström AC, Shah K et al. A recurrent gain of chromosome arm 3q in primary squamous carcinoma of the vagina. Cancer Genetics and Cytogenetics. 2004 Jan 1;148(1):7-13. https://doi.org/10.1016/S0165-4608(03)00245-0
Habermann, Jens K. ; Hellman, Kristina ; Freitag, Sandra ; Heselmeyer-Haddad, Kerstin ; Hellström, Ann Cathrin ; Shah, Keerti ; Auer, Gert ; Ried, Thomas. / A recurrent gain of chromosome arm 3q in primary squamous carcinoma of the vagina. In: Cancer Genetics and Cytogenetics. 2004 ; Vol. 148, No. 1. pp. 7-13.
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