A real-time PCR assay for rapid detection and quantification of Exserohilum rostratum, a causative pathogen of fungal meningitis associated with injection of contaminated methylprednisolone

Yanan Zhao, Ruta Petraitiene, Thomas J. Walsh, David S. Perlin

Research output: Contribution to journalArticlepeer-review

Abstract

A species-specific molecular beacon real-time PCR assay was developed for rapid diagnosis of Exserohilum rostratum infection. As low as 100 fg of E. rostratum DNA can be reliably detected in the presence of 50 ng of human DNA, with a dynamic linear quantification range from 20 ng to 200 fg.

Original languageEnglish (US)
Pages (from-to)1034-1036
Number of pages3
JournalJournal of clinical microbiology
Volume51
Issue number3
DOIs
StatePublished - Mar 2013

ASJC Scopus subject areas

  • Microbiology (medical)

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