A real-time PCR assay for rapid detection and quantification of Exserohilum rostratum, a causative pathogen of fungal meningitis associated with injection of contaminated methylprednisolone

Yanan Zhao; Ruta Petraitiene; Thomas J. Walsh; David S. Perlin

doi:10.1128/JCM.03369-12

A real-time PCR assay for rapid detection and quantification of Exserohilum rostratum, a causative pathogen of fungal meningitis associated with injection of contaminated methylprednisolone

Yanan Zhao, Ruta Petraitiene, Thomas J. Walsh, David S. Perlin

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

A species-specific molecular beacon real-time PCR assay was developed for rapid diagnosis of Exserohilum rostratum infection. As low as 100 fg of E. rostratum DNA can be reliably detected in the presence of 50 ng of human DNA, with a dynamic linear quantification range from 20 ng to 200 fg.

Original language	English (US)
Pages (from-to)	1034-1036
Number of pages	3
Journal	Journal of clinical microbiology
Volume	51
Issue number	3
DOIs	https://doi.org/10.1128/JCM.03369-12
State	Published - Mar 2013
Externally published	Yes

ASJC Scopus subject areas

Microbiology (medical)

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10.1128/JCM.03369-12

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A real-time PCR assay for rapid detection and quantification of Exserohilum rostratum, a causative pathogen of fungal meningitis associated with injection of contaminated methylprednisolone. / Zhao, Yanan; Petraitiene, Ruta; Walsh, Thomas J. et al.
In: Journal of clinical microbiology, Vol. 51, No. 3, 03.2013, p. 1034-1036.

Research output: Contribution to journal › Article › peer-review

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A real-time PCR assay for rapid detection and quantification of Exserohilum rostratum, a causative pathogen of fungal meningitis associated with injection of contaminated methylprednisolone

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