A rapidly rearranging retrotransposon within the miniexon gene locus of Crithidia fasciculata

Abram Gabriel, Tim J. Yen, David C. Schwartz, Cynthia L. Smith, Jef D. Boeke, Barbara Sollner-Webb, Don W. Cleveland

Research output: Contribution to journalArticle

Abstract

The tandemly arrayed miniexon genes of the trypanosomatid Crithidia fasciculata are interrupted at specific sites by multiple copies of an inserted element. The element, termed Crithidia retrotransposable element 1 (CRE1), is flanked by 29-base-pair target site duplications and contains a long 3′-terminal poly(dA) stretch. A single 1,140-codon reading frame is similar in sequence to the integrase and reverse transcriptase regions of retroviral pol polyproteins. Cloned lines derived from a stock of C. fasciculata have unique arrangements of CRE1s. In different cloned lines, CRE1s, in association with miniexon genes, are located on multiple chromosomes. By examining the arrangement of CRE1s in subclones, we estimate that the element rearranges at a rate of ca. 1% per generation. These results indicate that the C. fasciculata miniexon locus is the target for a novel retrotransposon.

Original languageEnglish (US)
Pages (from-to)615-624
Number of pages10
JournalMolecular and Cellular Biology
Volume10
Issue number2
StatePublished - Feb 1990

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Crithidia fasciculata
Retroelements
pol Gene Products
Crithidia
Genes
Integrases
Reading Frames
RNA-Directed DNA Polymerase
Codon
Base Pairing
Chromosomes

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Gabriel, A., Yen, T. J., Schwartz, D. C., Smith, C. L., Boeke, J. D., Sollner-Webb, B., & Cleveland, D. W. (1990). A rapidly rearranging retrotransposon within the miniexon gene locus of Crithidia fasciculata. Molecular and Cellular Biology, 10(2), 615-624.

A rapidly rearranging retrotransposon within the miniexon gene locus of Crithidia fasciculata. / Gabriel, Abram; Yen, Tim J.; Schwartz, David C.; Smith, Cynthia L.; Boeke, Jef D.; Sollner-Webb, Barbara; Cleveland, Don W.

In: Molecular and Cellular Biology, Vol. 10, No. 2, 02.1990, p. 615-624.

Research output: Contribution to journalArticle

Gabriel, A, Yen, TJ, Schwartz, DC, Smith, CL, Boeke, JD, Sollner-Webb, B & Cleveland, DW 1990, 'A rapidly rearranging retrotransposon within the miniexon gene locus of Crithidia fasciculata', Molecular and Cellular Biology, vol. 10, no. 2, pp. 615-624.
Gabriel A, Yen TJ, Schwartz DC, Smith CL, Boeke JD, Sollner-Webb B et al. A rapidly rearranging retrotransposon within the miniexon gene locus of Crithidia fasciculata. Molecular and Cellular Biology. 1990 Feb;10(2):615-624.
Gabriel, Abram ; Yen, Tim J. ; Schwartz, David C. ; Smith, Cynthia L. ; Boeke, Jef D. ; Sollner-Webb, Barbara ; Cleveland, Don W. / A rapidly rearranging retrotransposon within the miniexon gene locus of Crithidia fasciculata. In: Molecular and Cellular Biology. 1990 ; Vol. 10, No. 2. pp. 615-624.
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