A rapidly dividing human medulloblastoma cell line (D283 MED) express all three neurofilament subunits

J. Q. Trojanowski, H. S. Friedman, P. C. Burger, D. D. Bigner

Research output: Contribution to journalArticle

Abstract

The majority of cells in a rapidly dividing human medulloblastoma cell line (D283 MED) are shown to express the two high-molecular-weight human neurofilament (NF) subunits, whereas a minority express the low-molecular-weight NF subunit. These three polypeptides are integral subunits of the intermediate filaments (IFs) found in normal neurons. Other cell type-specific IF proteins (keratin, desmin, and glial filament polypeptides) are not present in D283 MED cells. Further, the immunocytochemical, immunochemical and ultrastructural data suggest that the neurofilaments in these cells are abnormal, possibly because of a paucity of the low-molecular-weight NF subunit. This is the first human cell line derived from a central nervous system tumor that is capable of expressing all three NF triplet proteins. It is a unique model system for studies of normal and abnormal human NF metabolism as well as for probing the cell biology of medulloblastomas.

Original languageEnglish (US)
Pages (from-to)358-363
Number of pages6
JournalAmerican Journal of Pathology
Volume126
Issue number2
StatePublished - 1987
Externally publishedYes

Fingerprint

Medulloblastoma
Intermediate Filaments
Cell Line
Molecular Weight
Neurofilament Proteins
Intermediate Filament Proteins
Central Nervous System Neoplasms
Peptides
Desmin
Keratins
Neuroglia
Cell Biology
Neurons

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Trojanowski, J. Q., Friedman, H. S., Burger, P. C., & Bigner, D. D. (1987). A rapidly dividing human medulloblastoma cell line (D283 MED) express all three neurofilament subunits. American Journal of Pathology, 126(2), 358-363.

A rapidly dividing human medulloblastoma cell line (D283 MED) express all three neurofilament subunits. / Trojanowski, J. Q.; Friedman, H. S.; Burger, P. C.; Bigner, D. D.

In: American Journal of Pathology, Vol. 126, No. 2, 1987, p. 358-363.

Research output: Contribution to journalArticle

Trojanowski, JQ, Friedman, HS, Burger, PC & Bigner, DD 1987, 'A rapidly dividing human medulloblastoma cell line (D283 MED) express all three neurofilament subunits', American Journal of Pathology, vol. 126, no. 2, pp. 358-363.
Trojanowski, J. Q. ; Friedman, H. S. ; Burger, P. C. ; Bigner, D. D. / A rapidly dividing human medulloblastoma cell line (D283 MED) express all three neurofilament subunits. In: American Journal of Pathology. 1987 ; Vol. 126, No. 2. pp. 358-363.
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