A rapid test for soy aeroallergens exposure assessment

Daniel Álvarez-Simon, María Jesús Cruz, María Dolores Untoria, Xavier Muñoz, Joan R. Villalbí, Ferran Morell, Susana Gómez-Ollés

Research output: Contribution to journalArticle

Abstract

Background: Determining soy aeroallergens levels is extremely important in the assessment of health risks due to these airborne substances. Currently, soy aeroallergens exposure in the environment is monitored using enzyme immunoassays (EIA) which must be evaluated in a specialized laboratory by skilled personnel. Objective: To describe the development and performance of a rapid immunochromatography assay for the detection of soy aeroallergens in environmental samples. Methods: A test strip using gold labeled anti-soy hull low molecular weight extract (SHLMWE) antibody for the rapid detection of soy aeroallergens in environmental samples was developed. One hundred nineteen airborne samples were analysed in parallel by the strip assay and the anti-SHLMWE sandwich EIA. The assay results were visually analysed by three independent observers who ranked samples as: -, + or ++. Strips were also scanned and analysed by densitometry. Results: The rapid test detected a range of concentrations from 6.25 to 25 ng/mL. Agreement in strip assay interpretations between evaluators was substantial (Kappa = 0.63; CI 0.544-0.715). Visual interpretation also gave a good concordance with EIA results, with sensitivity ranging from 77.3 to 100 and specificity from 65 to 83.5 depending on the observer. Furthermore, a strong correlation was observed between densitometry results of strip assay and EIA determinations. Conclusions: The strip assay developed is rapid, simple, and sensitive and does not require expensive equipment or specific skills. It has considerable potential in the environmental monitoring field for screening soy aeroallergens levels in port cities where allergen measurements are not currently performed. Due to its simplicity, the test will improve the management of soy allergic patients by controlling environmental allergen exposure without the need for apparatus or skilled personnel.

Original languageEnglish (US)
Article numbere88676
JournalPloS one
Volume9
Issue number2
DOIs
StatePublished - Feb 12 2014

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Fingerprint Dive into the research topics of 'A rapid test for soy aeroallergens exposure assessment'. Together they form a unique fingerprint.

  • Cite this

    Álvarez-Simon, D., Cruz, M. J., Untoria, M. D., Muñoz, X., Villalbí, J. R., Morell, F., & Gómez-Ollés, S. (2014). A rapid test for soy aeroallergens exposure assessment. PloS one, 9(2), [e88676]. https://doi.org/10.1371/journal.pone.0088676