TY - JOUR
T1 - A rapid method purifies a glycoprotein of Mr 145,000 as the LDL receptor of Trypanosoma brucei brucei
AU - Coppens, I.
AU - Bastin, Ph
AU - Courtoy, P. J.
AU - Baudhuin, P.
AU - Opperdoes, F. R.
N1 - Funding Information:
We thank J. Van Roy and D. Cottem for the isolation and the cultivation of trypanosomes. This work has been financially supported by the UNDP/World bank/WHO Special Programme for Research and Training in Tropical Diseases, by grant of the belgian Fonds National de la Recherche Scientifique at which IC is Senior Research Assistant and PJC Senior Research Associate, and by a fellowship of the belgian Institut pour 1’Encouragement de la Recherche Scientifique dans 1’Industrie et 1’Agriculture (to PhB).
PY - 1991/7/15
Y1 - 1991/7/15
N2 - The trypanosome LDL receptor has been isolated from bloodstream form and cultured insect-stage trypanosomes as a protein of Mr 145,000, using a rapid purification procedure in the presence of a cocktail of protease inhibitors, whereas previously a polypeptide of Mr 86,000 was purified as the LDL receptor. Both the 145,000 and the 86,000 polypeptides are glycosylated and recognized by a monospecific antibody raised against the 86,000 species. This antibody inhibits LDL binding to the intact trypanosomes, to the isolated 145,000 receptor and to the 86,000 species. Hence, the previously isolated 86,000 polypeptide is a degradation product probably representing the cleaved-off ectodomain of the trypanosome LDL receptor.
AB - The trypanosome LDL receptor has been isolated from bloodstream form and cultured insect-stage trypanosomes as a protein of Mr 145,000, using a rapid purification procedure in the presence of a cocktail of protease inhibitors, whereas previously a polypeptide of Mr 86,000 was purified as the LDL receptor. Both the 145,000 and the 86,000 polypeptides are glycosylated and recognized by a monospecific antibody raised against the 86,000 species. This antibody inhibits LDL binding to the intact trypanosomes, to the isolated 145,000 receptor and to the 86,000 species. Hence, the previously isolated 86,000 polypeptide is a degradation product probably representing the cleaved-off ectodomain of the trypanosome LDL receptor.
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U2 - 10.1016/0006-291X(91)91797-G
DO - 10.1016/0006-291X(91)91797-G
M3 - Article
C2 - 2069558
AN - SCOPUS:0025741446
SN - 0006-291X
VL - 178
SP - 185
EP - 191
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -