A rapid method for microarray cross platform comparisons using gene expression signatures

Chris Cheadle, Kevin G. Becker, Yoon S. Cho-Chung, Maria Nesterova, Tonya Watkins, William Wood, Vinayakumar Prabhu, Kathleen C. Barnes

Research output: Contribution to journalArticle

Abstract

Microarray technology has become highly valuable for identifying complex changes in global gene expression patterns. The inevitable use of a variety of different platforms has compounded the difficulty of effectively comparing data between projects, laboratories, and public access databases. The need for consistent, believable results across platforms is fundamental and methods for comparing results across platforms should be as straightforward as possible. We present the results of a study comparing three major, commercially available, microarray platforms (Affymetrix, Agilent, and Illumina). Concordance estimates between platforms was based on mapping of probes to Human Gene Organization (HUGO) gene names. Appropriate data normalization procedures were applied to each dataset followed by the generation of lists of regulated genes using a common significance threshold for all three platforms. As expected, concordance measured by directly comparing genelists was relatively low (an average 22.8% for all platforms across all possible comparisons). However, when statistical tests (gene set enrichment analysis-GSEA, parametric analysis of gene enrichment-PAGE) which align genelists with continuous measures of differential gene expression were applied to the cross platform datasets using significant genelists to poll entire datasets, the relatedness of the results from all three platforms was specific, obvious, and profound.

Original languageEnglish (US)
Pages (from-to)35-46
Number of pages12
JournalMolecular and Cellular Probes
Volume21
Issue number1
DOIs
Publication statusPublished - Feb 2007

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ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Cheadle, C., Becker, K. G., Cho-Chung, Y. S., Nesterova, M., Watkins, T., Wood, W., ... Barnes, K. C. (2007). A rapid method for microarray cross platform comparisons using gene expression signatures. Molecular and Cellular Probes, 21(1), 35-46. https://doi.org/10.1016/j.mcp.2006.07.004