TY - JOUR
T1 - A rapid growth-independent antibiotic resistance detection test by SYBR Green/Propidium Iodide viability assay
AU - Feng, Jie
AU - Yee, Rebecca
AU - Zhang, Shuo
AU - Tian, Lili
AU - Shi, Wanliang
AU - Zhang, Wen Hong
AU - Zhang, Ying
N1 - Publisher Copyright:
© 2018 Feng, Yee, Zhang, Tian, Shi, Zhang and Zhang.
PY - 2018/5/1
Y1 - 2018/5/1
N2 - Antibiotic-resistant bacteria have caused huge concerns and demand innovative approaches for their prompt detection. Current antimicrobial susceptibility tests (AST) rely on the growth of the organisms which takes 1-2 days for fast-growing organisms and several weeks for slow growing organisms. Here, we show for the first time the utility of the SYBR Green I/propidium iodide (PI) viability assay for rapidly identifying antibiotic resistance in less than 30 min for major, antibiotic-resistant, fast-growing bacteria, such as Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, and Acinetobacter baumannii for bactericidal and bacteriostatic agents and in 16 h for extremely rapid detection of drug resistance for isoniazid and pyrazinamide in slow-growing Mycobacterium tuberculosis. The SYBR Green I/PI assay generated rapid and robust results in concordance with traditional AST methods. This novel growth-independent methodology changes the concept of the current growth-based AST and may revolutionize current drug susceptibility testing for all cells of prokaryotic and eukaryotic origin and, subject to further clinical validation, may play a major role in saving lives and improving patient outcomes.
AB - Antibiotic-resistant bacteria have caused huge concerns and demand innovative approaches for their prompt detection. Current antimicrobial susceptibility tests (AST) rely on the growth of the organisms which takes 1-2 days for fast-growing organisms and several weeks for slow growing organisms. Here, we show for the first time the utility of the SYBR Green I/propidium iodide (PI) viability assay for rapidly identifying antibiotic resistance in less than 30 min for major, antibiotic-resistant, fast-growing bacteria, such as Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, and Acinetobacter baumannii for bactericidal and bacteriostatic agents and in 16 h for extremely rapid detection of drug resistance for isoniazid and pyrazinamide in slow-growing Mycobacterium tuberculosis. The SYBR Green I/PI assay generated rapid and robust results in concordance with traditional AST methods. This novel growth-independent methodology changes the concept of the current growth-based AST and may revolutionize current drug susceptibility testing for all cells of prokaryotic and eukaryotic origin and, subject to further clinical validation, may play a major role in saving lives and improving patient outcomes.
KW - Antibiotic resistance
KW - Antimicrobial susceptibility testing
KW - CRE bacteria
KW - Mycobacterium tuberculosis
KW - SYBR Green/PI availability assay
KW - Staphylococcus aureus
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U2 - 10.3389/fmed.2018.00127
DO - 10.3389/fmed.2018.00127
M3 - Article
C2 - 29774213
AN - SCOPUS:85050085119
SN - 2296-858X
VL - 5
JO - Frontiers in Medicine
JF - Frontiers in Medicine
IS - MAY
M1 - 127
ER -