A rapid growth-independent antibiotic resistance detection test by SYBR Green/Propidium Iodide viability assay

Jie Feng, Rebecca Yee, Shuo Zhang, Lili Tian, Wanliang Shi, Wen Hong Zhang, Ying Zhang

Research output: Contribution to journalArticle

Abstract

Antibiotic-resistant bacteria have caused huge concerns and demand innovative approaches for their prompt detection. Current antimicrobial susceptibility tests (AST) rely on the growth of the organisms which takes 1-2 days for fast-growing organisms and several weeks for slow growing organisms. Here, we show for the first time the utility of the SYBR Green I/propidium iodide (PI) viability assay for rapidly identifying antibiotic resistance in less than 30 min for major, antibiotic-resistant, fast-growing bacteria, such as Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, and Acinetobacter baumannii for bactericidal and bacteriostatic agents and in 16 h for extremely rapid detection of drug resistance for isoniazid and pyrazinamide in slow-growing Mycobacterium tuberculosis. The SYBR Green I/PI assay generated rapid and robust results in concordance with traditional AST methods. This novel growth-independent methodology changes the concept of the current growth-based AST and may revolutionize current drug susceptibility testing for all cells of prokaryotic and eukaryotic origin and, subject to further clinical validation, may play a major role in saving lives and improving patient outcomes.

Original languageEnglish (US)
Article number127
JournalFrontiers in Medicine
Volume5
Issue numberMAY
DOIs
StatePublished - May 1 2018

Fingerprint

Propidium
Microbial Drug Resistance
Growth
Prokaryotic Cells
Anti-Bacterial Agents
Bacteria
Pyrazinamide
Acinetobacter baumannii
Isoniazid
Klebsiella pneumoniae
Eukaryotic Cells
Mycobacterium tuberculosis
Drug Resistance
Staphylococcus aureus
Escherichia coli
Pharmaceutical Preparations
SYBR Green I

Keywords

  • Antibiotic resistance
  • Antimicrobial susceptibility testing
  • CRE bacteria
  • Mycobacterium tuberculosis
  • Staphylococcus aureus
  • SYBR Green/PI availability assay

ASJC Scopus subject areas

  • Medicine(all)

Cite this

A rapid growth-independent antibiotic resistance detection test by SYBR Green/Propidium Iodide viability assay. / Feng, Jie; Yee, Rebecca; Zhang, Shuo; Tian, Lili; Shi, Wanliang; Zhang, Wen Hong; Zhang, Ying.

In: Frontiers in Medicine, Vol. 5, No. MAY, 127, 01.05.2018.

Research output: Contribution to journalArticle

@article{b684470c948040358d8384d6c6789d00,
title = "A rapid growth-independent antibiotic resistance detection test by SYBR Green/Propidium Iodide viability assay",
abstract = "Antibiotic-resistant bacteria have caused huge concerns and demand innovative approaches for their prompt detection. Current antimicrobial susceptibility tests (AST) rely on the growth of the organisms which takes 1-2 days for fast-growing organisms and several weeks for slow growing organisms. Here, we show for the first time the utility of the SYBR Green I/propidium iodide (PI) viability assay for rapidly identifying antibiotic resistance in less than 30 min for major, antibiotic-resistant, fast-growing bacteria, such as Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, and Acinetobacter baumannii for bactericidal and bacteriostatic agents and in 16 h for extremely rapid detection of drug resistance for isoniazid and pyrazinamide in slow-growing Mycobacterium tuberculosis. The SYBR Green I/PI assay generated rapid and robust results in concordance with traditional AST methods. This novel growth-independent methodology changes the concept of the current growth-based AST and may revolutionize current drug susceptibility testing for all cells of prokaryotic and eukaryotic origin and, subject to further clinical validation, may play a major role in saving lives and improving patient outcomes.",
keywords = "Antibiotic resistance, Antimicrobial susceptibility testing, CRE bacteria, Mycobacterium tuberculosis, Staphylococcus aureus, SYBR Green/PI availability assay",
author = "Jie Feng and Rebecca Yee and Shuo Zhang and Lili Tian and Wanliang Shi and Zhang, {Wen Hong} and Ying Zhang",
year = "2018",
month = "5",
day = "1",
doi = "10.3389/fmed.2018.00127",
language = "English (US)",
volume = "5",
journal = "Frontiers in Medicine",
issn = "2296-858X",
publisher = "Frontiers Media S. A.",
number = "MAY",

}

TY - JOUR

T1 - A rapid growth-independent antibiotic resistance detection test by SYBR Green/Propidium Iodide viability assay

AU - Feng, Jie

AU - Yee, Rebecca

AU - Zhang, Shuo

AU - Tian, Lili

AU - Shi, Wanliang

AU - Zhang, Wen Hong

AU - Zhang, Ying

PY - 2018/5/1

Y1 - 2018/5/1

N2 - Antibiotic-resistant bacteria have caused huge concerns and demand innovative approaches for their prompt detection. Current antimicrobial susceptibility tests (AST) rely on the growth of the organisms which takes 1-2 days for fast-growing organisms and several weeks for slow growing organisms. Here, we show for the first time the utility of the SYBR Green I/propidium iodide (PI) viability assay for rapidly identifying antibiotic resistance in less than 30 min for major, antibiotic-resistant, fast-growing bacteria, such as Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, and Acinetobacter baumannii for bactericidal and bacteriostatic agents and in 16 h for extremely rapid detection of drug resistance for isoniazid and pyrazinamide in slow-growing Mycobacterium tuberculosis. The SYBR Green I/PI assay generated rapid and robust results in concordance with traditional AST methods. This novel growth-independent methodology changes the concept of the current growth-based AST and may revolutionize current drug susceptibility testing for all cells of prokaryotic and eukaryotic origin and, subject to further clinical validation, may play a major role in saving lives and improving patient outcomes.

AB - Antibiotic-resistant bacteria have caused huge concerns and demand innovative approaches for their prompt detection. Current antimicrobial susceptibility tests (AST) rely on the growth of the organisms which takes 1-2 days for fast-growing organisms and several weeks for slow growing organisms. Here, we show for the first time the utility of the SYBR Green I/propidium iodide (PI) viability assay for rapidly identifying antibiotic resistance in less than 30 min for major, antibiotic-resistant, fast-growing bacteria, such as Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, and Acinetobacter baumannii for bactericidal and bacteriostatic agents and in 16 h for extremely rapid detection of drug resistance for isoniazid and pyrazinamide in slow-growing Mycobacterium tuberculosis. The SYBR Green I/PI assay generated rapid and robust results in concordance with traditional AST methods. This novel growth-independent methodology changes the concept of the current growth-based AST and may revolutionize current drug susceptibility testing for all cells of prokaryotic and eukaryotic origin and, subject to further clinical validation, may play a major role in saving lives and improving patient outcomes.

KW - Antibiotic resistance

KW - Antimicrobial susceptibility testing

KW - CRE bacteria

KW - Mycobacterium tuberculosis

KW - Staphylococcus aureus

KW - SYBR Green/PI availability assay

UR - http://www.scopus.com/inward/record.url?scp=85050085119&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85050085119&partnerID=8YFLogxK

U2 - 10.3389/fmed.2018.00127

DO - 10.3389/fmed.2018.00127

M3 - Article

C2 - 29774213

AN - SCOPUS:85050085119

VL - 5

JO - Frontiers in Medicine

JF - Frontiers in Medicine

SN - 2296-858X

IS - MAY

M1 - 127

ER -