A rapid extraction technique for atomic absorption determinations of kidney calcium

William P. Tew, Charles D. Malis, W. Gordon Walker

Research output: Contribution to journalArticle

Abstract

A simple method of extracting calcium from kidney tissue prior to atomic absorption measurements of calcium in experimental models of nephrocalcinosis is described. This technique results in substantial time savings over traditional ashing or acid-digestion techniques for monitoring the rate and extent of calcification of the kidney. Comparisons of calcium in the kidneys of mice maintained on regimens which produce both intra- and extracellular calcification by both extraction and ashing techniques produced statistically equivalent results. Brief sonication of the tissue in a butanol-HCl-lanthanum medium effectively extracted calcium allowing accurate determination of tissue calcium without ashing. This technique has proven very useful when numerous samples must be processed daily.

Original languageEnglish (US)
Pages (from-to)346-350
Number of pages5
JournalAnalytical biochemistry
Volume112
Issue number2
DOIs
StatePublished - Apr 1981

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ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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