A rapid extraction technique for atomic absorption determinations of kidney calcium

William P. Tew; Charles D. Malis; W. Gordon Walker

doi:10.1016/0003-2697(81)90303-1

A rapid extraction technique for atomic absorption determinations of kidney calcium

William P. Tew, Charles D. Malis, W. Gordon Walker

School of Medicine

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

A simple method of extracting calcium from kidney tissue prior to atomic absorption measurements of calcium in experimental models of nephrocalcinosis is described. This technique results in substantial time savings over traditional ashing or acid-digestion techniques for monitoring the rate and extent of calcification of the kidney. Comparisons of calcium in the kidneys of mice maintained on regimens which produce both intra- and extracellular calcification by both extraction and ashing techniques produced statistically equivalent results. Brief sonication of the tissue in a butanol-HCl-lanthanum medium effectively extracted calcium allowing accurate determination of tissue calcium without ashing. This technique has proven very useful when numerous samples must be processed daily.

Original language	English (US)
Pages (from-to)	346-350
Number of pages	5
Journal	Analytical biochemistry
Volume	112
Issue number	2
DOIs	https://doi.org/10.1016/0003-2697(81)90303-1
State	Published - Apr 1981

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0003-2697(81)90303-1

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@article{6e3016061ab04852aee79d7dcac093e2,

title = "A rapid extraction technique for atomic absorption determinations of kidney calcium",

abstract = "A simple method of extracting calcium from kidney tissue prior to atomic absorption measurements of calcium in experimental models of nephrocalcinosis is described. This technique results in substantial time savings over traditional ashing or acid-digestion techniques for monitoring the rate and extent of calcification of the kidney. Comparisons of calcium in the kidneys of mice maintained on regimens which produce both intra- and extracellular calcification by both extraction and ashing techniques produced statistically equivalent results. Brief sonication of the tissue in a butanol-HCl-lanthanum medium effectively extracted calcium allowing accurate determination of tissue calcium without ashing. This technique has proven very useful when numerous samples must be processed daily.",

author = "Tew, {William P.} and Malis, {Charles D.} and Walker, {W. Gordon}",

note = "Funding Information: The authors wish to thank Ms. Jane Foard for expert technical assistance. This work was supported by grants from the Harris Foundation and the Blum Foundation. William P. Tew was supported by NFS Grant PCM-7818190 and NIH Grant GM05919 and by a grant from the Harris Foundation; Charles D. Malis by NIH Training Grant S-T-32-AM-07145. Computational assistance was received from CLINFO, sponsored by NIH Grant 5MOlRR35-20.",

year = "1981",

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doi = "10.1016/0003-2697(81)90303-1",

language = "English (US)",

volume = "112",

pages = "346--350",

journal = "Analytical biochemistry",

issn = "0003-2697",

publisher = "Academic Press Inc.",

number = "2",

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TY - JOUR

T1 - A rapid extraction technique for atomic absorption determinations of kidney calcium

AU - Tew, William P.

AU - Malis, Charles D.

AU - Walker, W. Gordon

N1 - Funding Information: The authors wish to thank Ms. Jane Foard for expert technical assistance. This work was supported by grants from the Harris Foundation and the Blum Foundation. William P. Tew was supported by NFS Grant PCM-7818190 and NIH Grant GM05919 and by a grant from the Harris Foundation; Charles D. Malis by NIH Training Grant S-T-32-AM-07145. Computational assistance was received from CLINFO, sponsored by NIH Grant 5MOlRR35-20.

PY - 1981/4

Y1 - 1981/4

N2 - A simple method of extracting calcium from kidney tissue prior to atomic absorption measurements of calcium in experimental models of nephrocalcinosis is described. This technique results in substantial time savings over traditional ashing or acid-digestion techniques for monitoring the rate and extent of calcification of the kidney. Comparisons of calcium in the kidneys of mice maintained on regimens which produce both intra- and extracellular calcification by both extraction and ashing techniques produced statistically equivalent results. Brief sonication of the tissue in a butanol-HCl-lanthanum medium effectively extracted calcium allowing accurate determination of tissue calcium without ashing. This technique has proven very useful when numerous samples must be processed daily.

AB - A simple method of extracting calcium from kidney tissue prior to atomic absorption measurements of calcium in experimental models of nephrocalcinosis is described. This technique results in substantial time savings over traditional ashing or acid-digestion techniques for monitoring the rate and extent of calcification of the kidney. Comparisons of calcium in the kidneys of mice maintained on regimens which produce both intra- and extracellular calcification by both extraction and ashing techniques produced statistically equivalent results. Brief sonication of the tissue in a butanol-HCl-lanthanum medium effectively extracted calcium allowing accurate determination of tissue calcium without ashing. This technique has proven very useful when numerous samples must be processed daily.

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JF - Analytical biochemistry

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ER -

A rapid extraction technique for atomic absorption determinations of kidney calcium

Abstract

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