Abstract
Recently we have found that W-Beijing Mycobacterium tuberculosis strains have a unique in-frame trinucleotide (AGC) deletion at position 421 of Rv0927c and a -127G → A mutation in Rv0927c-pstS3 intergenic region. Based on detecting the 421 trinucleotide deletion of these two mutations which can alter the ssDNA conformation more extensively than the other, we developed a PCR-SSCP method for rapid identification of W-Beijing strains among non-Beijing strains. Altogether, 104 clinical isolates were analyzed, including 68 W-Beijing strains and 36 non-Beijing strains. We found that PCR-SSCP successfully differentiated all the W-Beijing strains from the non-Beijing strains. In addition, we unexpectedly discovered that SDS-PAGE protein gels had better resolving power than conventional TBE polyacrylamide gel in detecting the AGC deletion mutation in the SSCP analysis.
Original language | English (US) |
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Pages (from-to) | 419-423 |
Number of pages | 5 |
Journal | Microbes and Infection |
Volume | 11 |
Issue number | 3 |
DOIs | |
State | Published - Mar 2009 |
Keywords
- Mycobacterium tuberculosis
- PCR-SSCP
- Rv0927c
- Strain identification
- W-Beijing strain
ASJC Scopus subject areas
- Microbiology
- Immunology
- Infectious Diseases