A rapid and potent DNA vaccination strategy defined by in vivo monitoring of antigen expression

Adriaan D. Bins, Annelies Jorritsma, Monika C. Wolkers, Chien Fu Hung, T. C. Wu, Ton N.M. Schumacher, John B.A.G. Haanen

Research output: Contribution to journalArticlepeer-review

Abstract

Induction of immunity after DNA vaccination is generally considered a slow process. Here we show that DNA delivery to the skin results in a highly transient pulse of antigen expression. Based on this information, we developed a new rapid and potent intradermal DNA vaccination method. By short-interval intradermal DNA delivery, robust T-cell responses, of a magnitude sufficient to reject established subcutaneous tumors, are generated within 12 d. Moreover, this vaccination strategy confers protecting humoral immunity against influenza A infection within 2 weeks after the start of vaccination. The strength and speed of this newly developed strategy will be beneficial in situations in which immunity is required in the shortest possible time.

Original languageEnglish (US)
Pages (from-to)899-904
Number of pages6
JournalNature medicine
Volume11
Issue number8
DOIs
StatePublished - Aug 2005

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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