A rapid and fully-automated method for the quantitation of tricyclic antidepressants in serum using turbulent-flow liquid chromatography-tandem mass spectrometry.

Autumn R. Breaud, Robert Harlan, Joseph M. Di Bussolo, Gwendolyn A. McMillin, William Clarke

Research output: Contribution to journalArticle

Abstract

We describe a fully-automated turbulent-flow liquid chromatography-tandem mass spectrometry method for the detection of tricyclic antidepressant drugs (amitriptyline, desipramine, imipramine, and nortriptyline) in serum. Human serum and an internal standard were injected directly onto a Cyclone-P online solid-phase extraction (SPE) column (0.5 x 50 mm). Following removal of serum proteins and other components the analytes were transferred to a Hypersil Gold C-18 (50 x 3 mm) analytical column. Elution occurred with a gradient of water and acetonitrile each with 0.1% formic acid. Analytes were ionized and detected over a 3.5 min analysis time by electrospray-ionization mass spectrometry with selected reaction monitoring (SRM). Matrix effects were well-characterized and carryover, precision, linearity, recovery and limits of detection and quantitation were evaluated. The simple and complex precision CVs for all compounds were <or = 16%. The limits of detection and quantitation for all drugs were <or = 3 ng/ml and 0.96. Proficiency testing materials had values within 2 SDI of peer group means for all drugs. Based on validation data, this is a specific, sensitive fully-automated method for rapid quantitation of tricyclic antidepressants in serum.

Original languageEnglish (US)
Pages (from-to)825-832
Number of pages8
JournalClinica Chimica Acta
Volume411
Issue number11-12
StatePublished - Jun 3 2010
Externally publishedYes

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formic acid
Tricyclic Antidepressive Agents
Liquid chromatography
Tandem Mass Spectrometry
Liquid Chromatography
Turbulent flow
Mass spectrometry
Nortriptyline
Electrospray ionization
Desipramine
Amitriptyline
Materials testing
Limit of Detection
Imipramine
Serum
Gold
Pharmaceutical Preparations
Materials Testing
Cyclonic Storms
Blood Proteins

ASJC Scopus subject areas

  • Medicine(all)

Cite this

A rapid and fully-automated method for the quantitation of tricyclic antidepressants in serum using turbulent-flow liquid chromatography-tandem mass spectrometry. / Breaud, Autumn R.; Harlan, Robert; Di Bussolo, Joseph M.; McMillin, Gwendolyn A.; Clarke, William.

In: Clinica Chimica Acta, Vol. 411, No. 11-12, 03.06.2010, p. 825-832.

Research output: Contribution to journalArticle

Breaud, Autumn R. ; Harlan, Robert ; Di Bussolo, Joseph M. ; McMillin, Gwendolyn A. ; Clarke, William. / A rapid and fully-automated method for the quantitation of tricyclic antidepressants in serum using turbulent-flow liquid chromatography-tandem mass spectrometry. In: Clinica Chimica Acta. 2010 ; Vol. 411, No. 11-12. pp. 825-832.
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