A radiolabeled antiglobulin test for crossmatching platelet transfusions

Thomas Stephen Kickler, H. G. Braine, Paul Michael Ness, A. Koester, W. Bias

Research output: Contribution to journalArticle

Abstract

Despite the use of HLA-matched platelets for alloimmunized recipients, transfusion failures occur. In order to reduce these failures, we investigated the use of a radiolabeled antiglobulin technique for platelet crossmatching. The principle of the test is that of an indirect Coombs test using 125I labeled goat anti-human IgG. Incompatibility is determined by calculating a radioactivity antiglobulin test (RAGT) index. Using this technique, we performed 89 crossmatches on 19 leukemic or aplastic patients who were refractory to random donor platelets and receiving varying degrees of HLA-matched platelets. Effectiveness of the transfusion was assessed from the posttransfusion corrected platelet count increment (CCI) determined at 1 and 20 hr. When the RAGT index was 1.9 or less, the mean CCI at 1 hr was 17,570 ± 7003/cu mm, n = 55. When the RAGT index was 2.0 or greater, the mean CCI was 4237 ± 4100/cu mm, n = 34. At 20 hr when the RAGT index was 1.9 or less, the mean CCI was 8722 ± 3143/cu mm, n = 33, and when the index was 2.0 or greater, the mean CCI was 571 ± 1286/cu mm, n = 23. Using this technique, one false negative resulted. Nine positive crossmatches with good increments at 1 hr were found; at 20 hr, however, the survival of these units was zero. These data suggest that this method is a useful adjunct in the selection of platelets in the refractory patient.

Original languageEnglish (US)
Pages (from-to)238-242
Number of pages5
JournalBlood
Volume61
Issue number2
StatePublished - 1983

Fingerprint

Coombs Test
Platelet Transfusion
Platelets
Platelet Count
Anti-Idiotypic Antibodies
Blood Platelets
Radioactivity
Goats
Refractory materials
Tissue Donors
Survival
Immunoglobulin G

ASJC Scopus subject areas

  • Hematology

Cite this

A radiolabeled antiglobulin test for crossmatching platelet transfusions. / Kickler, Thomas Stephen; Braine, H. G.; Ness, Paul Michael; Koester, A.; Bias, W.

In: Blood, Vol. 61, No. 2, 1983, p. 238-242.

Research output: Contribution to journalArticle

Kickler, TS, Braine, HG, Ness, PM, Koester, A & Bias, W 1983, 'A radiolabeled antiglobulin test for crossmatching platelet transfusions', Blood, vol. 61, no. 2, pp. 238-242.
Kickler, Thomas Stephen ; Braine, H. G. ; Ness, Paul Michael ; Koester, A. ; Bias, W. / A radiolabeled antiglobulin test for crossmatching platelet transfusions. In: Blood. 1983 ; Vol. 61, No. 2. pp. 238-242.
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