A radioisotopic assay for proline oxidase activity

James M. Phang, Sylvia J. Downing, David L. Valle, Edward M. Kowaloff

Research output: Contribution to journalArticle

Abstract

We developed a radioisotopic assay for proline oxidase in which product Δ1-pyrroline-5-carboxylate-14C is reacted with o-aminobenzaldehyde and the radioactivity trapped as the dihydroquinazolinium compound is recovered by ion-exchange chromatography. The sensitivity of this method allows the measurement of proline oxidase activity in small specimens (10 to 20 mg.) of liver.

Original languageEnglish (US)
Pages (from-to)312-317
Number of pages6
JournalThe Journal of laboratory and clinical medicine
Volume85
Issue number2
StatePublished - Feb 1975

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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  • Cite this

    Phang, J. M., Downing, S. J., Valle, D. L., & Kowaloff, E. M. (1975). A radioisotopic assay for proline oxidase activity. The Journal of laboratory and clinical medicine, 85(2), 312-317.