A radioisotopic assay for ornithine-δ-transaminase

James M. Phang, Sylvia J. Downing, David Valle

Research output: Contribution to journalArticlepeer-review

Abstract

We describe a radioisotopic assay for ornithine-δ-transaminase using precursor ornithine-U-14C. We quantitate the product Δ1-pyrroline-5-carboxylate-14C by cation-exchange chromatography. The sensitivity of the method allows for measurement of enzyme activity in extracts prepared from 105 mammalian cells.

Original languageEnglish (US)
Pages (from-to)272-277
Number of pages6
JournalAnalytical biochemistry
Volume55
Issue number1
DOIs
StatePublished - Sep 1973
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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