A radioisotopic assay for Δ1-pyrroline-5-carboxylate reductase

James M. Phang; Sylvia J. Downing; David Valle

doi:10.1016/0003-2697(73)90312-6

A radioisotopic assay for Δ¹-pyrroline-5-carboxylate reductase

James M. Phang, Sylvia J. Downing, David Valle

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

We describe a radioisotopic assay for Δ¹-pyrroline-5-carboxylate reductase. In this assay we use Δ¹-pyrroline-5-carboxylate[U-¹⁴C] and isolate product l-[U-¹⁴C]proline by cation-exchange column chromatography.

Original language	English (US)
Pages (from-to)	266-271
Number of pages	6
Journal	Analytical biochemistry
Volume	55
Issue number	1
DOIs	https://doi.org/10.1016/0003-2697(73)90312-6
State	Published - Sep 1973
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0003-2697(73)90312-6

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@article{1a2391061120403da778e468df313afc,

title = "A radioisotopic assay for Δ1-pyrroline-5-carboxylate reductase",

abstract = "We describe a radioisotopic assay for Δ1-pyrroline-5-carboxylate reductase. In this assay we use Δ1-pyrroline-5-carboxylate[U-14C] and isolate product l-[U-14C]proline by cation-exchange column chromatography.",

author = "Phang, {James M.} and Downing, {Sylvia J.} and David Valle",

year = "1973",

month = sep,

doi = "10.1016/0003-2697(73)90312-6",

language = "English (US)",

volume = "55",

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journal = "Analytical biochemistry",

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publisher = "Academic Press Inc.",

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AU - Phang, James M.

AU - Downing, Sylvia J.

AU - Valle, David

PY - 1973/9

Y1 - 1973/9

N2 - We describe a radioisotopic assay for Δ1-pyrroline-5-carboxylate reductase. In this assay we use Δ1-pyrroline-5-carboxylate[U-14C] and isolate product l-[U-14C]proline by cation-exchange column chromatography.

AB - We describe a radioisotopic assay for Δ1-pyrroline-5-carboxylate reductase. In this assay we use Δ1-pyrroline-5-carboxylate[U-14C] and isolate product l-[U-14C]proline by cation-exchange column chromatography.

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UR - http://www.scopus.com/inward/citedby.url?scp=0015698158&partnerID=8YFLogxK

U2 - 10.1016/0003-2697(73)90312-6

DO - 10.1016/0003-2697(73)90312-6

M3 - Article

C2 - 4753151

AN - SCOPUS:0015698158

SN - 0003-2697

VL - 55

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EP - 271

JO - Analytical biochemistry

JF - Analytical biochemistry

IS - 1

ER -

A radioisotopic assay for Δ¹-pyrroline-5-carboxylate reductase

Abstract

ASJC Scopus subject areas

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