A rabbit model of non-typhoidal Salmonella bacteremia

Aruna Panda, Ivan Tatarov, Billie Jo Masek, Justin Hardick, Annabelle Crusan, Teresa Wakefield, Karen C Carroll, Samuel Yang, Yu-Hsiang Hsieh, Michael M. Lipsky, Charles G. McLeod, Myron M. Levine, Richard Rothman, Charlotte A Gaydos, Louis J. DeTolla

Research output: Contribution to journalArticle

Abstract

Bacteremia is an important cause of morbidity and mortality in humans. In this study, we focused on the development of an animal model of bacteremia induced by non-typhoidal Salmonella. New Zealand White rabbits were inoculated with a human isolate of non-typhoidal Salmonella strain CVD J73 via the intra-peritoneal route. Blood samples were collected at specific time points and at euthanasia from infected rabbits. Additionally, tissue samples from the heart, lungs, spleen, gastrointestinal tract, liver and kidneys were obtained at euthanasia. All experimentally infected rabbits displayed clinical signs of disease (fever, dehydration, weight loss and lethargy). Tissues collected at necropsy from the animals exhibited histopathological changes indicative of bacteremia. Non-typhoidal Salmonella bacteria were detected in the blood and tissue samples of infected rabbits by microbiological culture and real-time PCR assays. The development of this animal model of bacteremia could prove to be a useful tool for studying how non-typhoidal Salmonella infections disseminate and spread in humans.

Original languageEnglish (US)
Pages (from-to)211-220
Number of pages10
JournalComparative Immunology, Microbiology and Infectious Diseases
Volume37
Issue number4
DOIs
StatePublished - Sep 1 2014

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Keywords

  • Bacteremia
  • Non-typhoidal Salmonella infections
  • Rabbit model

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Infectious Diseases
  • Immunology and Allergy
  • veterinary(all)
  • Medicine(all)

Cite this

Panda, A., Tatarov, I., Masek, B. J., Hardick, J., Crusan, A., Wakefield, T., Carroll, K. C., Yang, S., Hsieh, Y-H., Lipsky, M. M., McLeod, C. G., Levine, M. M., Rothman, R., Gaydos, C. A., & DeTolla, L. J. (2014). A rabbit model of non-typhoidal Salmonella bacteremia. Comparative Immunology, Microbiology and Infectious Diseases, 37(4), 211-220. https://doi.org/10.1016/j.cimid.2014.05.004