A quantum dot based nanoassay for quantifying gene copy number with ultrahigh resolution

Yi Zhang, Ie Ming Shih, Tian-Li Wang, Tza Huei Wang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

DNA CNV is fundamental to study molecular etiology of human diseases such as developmental disorder and cancer [1, 2]. The current CNV detection relies on hybridization or semi quantitative PCR whose technical limitations compromise the accuracy. In addition, current techniques are not able to reliably differentiate less than a twofold change in CNV [3,4]. This paper presents a novel gene copy number variation (CNV) detection method named quantum dot (QD) Electrophoretic Mobility Shift Assay (QEMSA). This nanoassay demonstrates a remarkable resolution, capable of distinguishing a 1.1-fold (∼9%) difference in target copy number.

Original languageEnglish (US)
Title of host publication14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Pages1154-1156
Number of pages3
Volume2
StatePublished - 2010
Event14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 - Groningen, Netherlands
Duration: Oct 3 2010Oct 7 2010

Other

Other14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
CountryNetherlands
CityGroningen
Period10/3/1010/7/10

Fingerprint

Electrophoretic mobility
Semiconductor quantum dots
Assays
DNA
Genes

Keywords

  • Copy number variation
  • Nanoassay
  • Quantum dot
  • Ultrahigh resolution

ASJC Scopus subject areas

  • Control and Systems Engineering

Cite this

Zhang, Y., Shih, I. M., Wang, T-L., & Wang, T. H. (2010). A quantum dot based nanoassay for quantifying gene copy number with ultrahigh resolution. In 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 (Vol. 2, pp. 1154-1156)

A quantum dot based nanoassay for quantifying gene copy number with ultrahigh resolution. / Zhang, Yi; Shih, Ie Ming; Wang, Tian-Li; Wang, Tza Huei.

14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. Vol. 2 2010. p. 1154-1156.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Zhang, Y, Shih, IM, Wang, T-L & Wang, TH 2010, A quantum dot based nanoassay for quantifying gene copy number with ultrahigh resolution. in 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. vol. 2, pp. 1154-1156, 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010, Groningen, Netherlands, 10/3/10.
Zhang Y, Shih IM, Wang T-L, Wang TH. A quantum dot based nanoassay for quantifying gene copy number with ultrahigh resolution. In 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. Vol. 2. 2010. p. 1154-1156
Zhang, Yi ; Shih, Ie Ming ; Wang, Tian-Li ; Wang, Tza Huei. / A quantum dot based nanoassay for quantifying gene copy number with ultrahigh resolution. 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. Vol. 2 2010. pp. 1154-1156
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