A quantum dot based nanoassay for quantifying gene copy number with ultrahigh resolution

Yi Zhang, Ie Ming Shih, Tian Li Wang, Tza Huei Wang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

DNA CNV is fundamental to study molecular etiology of human diseases such as developmental disorder and cancer [1, 2]. The current CNV detection relies on hybridization or semi quantitative PCR whose technical limitations compromise the accuracy. In addition, current techniques are not able to reliably differentiate less than a twofold change in CNV [3,4]. This paper presents a novel gene copy number variation (CNV) detection method named quantum dot (QD) Electrophoretic Mobility Shift Assay (QEMSA). This nanoassay demonstrates a remarkable resolution, capable of distinguishing a 1.1-fold (∼9%) difference in target copy number.

Original languageEnglish (US)
Title of host publication14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Pages1154-1156
Number of pages3
StatePublished - Dec 1 2010
Event14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 - Groningen, Netherlands
Duration: Oct 3 2010Oct 7 2010

Publication series

Name14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Volume2

Other

Other14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
CountryNetherlands
CityGroningen
Period10/3/1010/7/10

Keywords

  • Copy number variation
  • Nanoassay
  • Quantum dot
  • Ultrahigh resolution

ASJC Scopus subject areas

  • Control and Systems Engineering

Fingerprint Dive into the research topics of 'A quantum dot based nanoassay for quantifying gene copy number with ultrahigh resolution'. Together they form a unique fingerprint.

Cite this