A quantitative system for studying metastasis using transparent zebrafish

Silja Heilmann, Kajan Ratnakumar, Erin M. Langdon, Emily R. Kansler, Isabella S. Kim, Nathaniel R. Campbell, Elizabeth B. Perry, Amy J. McMahon, Charles K. Kaufman, Ellen Van Rooijen, William Lee, Christine A. Iacobuzio-Donahue, Richard O. Hynes, Leonard I. Zon, Joao B. Xavier, Richard M. White

Research output: Contribution to journalArticle

Abstract

Metastasis is the defining feature of advanced malignancy, yet remains challenging to study in laboratory environments. Here, we describe a high-throughput zebrafish system for comprehensive, in vivo assessment of metastatic biology. First, we generated several stable cell lines from melanomas of transgenic mitfa-BRAFV600E;p53-/- fish. We then transplanted the melanoma cells into the transparent casper strain to enable highly quantitative measurement of the metastatic process at single-cell resolution. Using computational image analysis of the resulting metastases, we generated a metastasis score, μ, that can be applied to quantitative comparison of metastatic capacity between experimental conditions. Furthermore, image analysis also provided estimates of the frequency of metastasis-initiating cells (∼1/120,000 cells). Finally, we determined that the degree of pigmentation is a key feature defining cells with metastatic capability. The small size and rapid generation of progeny combined with superior imaging tools make zebrafish ideal for unbiased high-throughput investigations of cell-intrinsic or microenvironmental modifiers of metastasis. The approaches described here are readily applicable to other tumor types and thus serve to complement studies also employing murine and human cell culture systems.

Original languageEnglish (US)
Pages (from-to)4272-4282
Number of pages11
JournalCancer Research
Volume75
Issue number20
DOIs
StatePublished - Oct 15 2015
Externally publishedYes

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Zebrafish
Neoplasm Metastasis
Melanoma
Pigmentation
Neoplasms
Fishes
Cell Culture Techniques
Cell Line

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Heilmann, S., Ratnakumar, K., Langdon, E. M., Kansler, E. R., Kim, I. S., Campbell, N. R., ... White, R. M. (2015). A quantitative system for studying metastasis using transparent zebrafish. Cancer Research, 75(20), 4272-4282. https://doi.org/10.1158/0008-5472.CAN-14-3319

A quantitative system for studying metastasis using transparent zebrafish. / Heilmann, Silja; Ratnakumar, Kajan; Langdon, Erin M.; Kansler, Emily R.; Kim, Isabella S.; Campbell, Nathaniel R.; Perry, Elizabeth B.; McMahon, Amy J.; Kaufman, Charles K.; Van Rooijen, Ellen; Lee, William; Iacobuzio-Donahue, Christine A.; Hynes, Richard O.; Zon, Leonard I.; Xavier, Joao B.; White, Richard M.

In: Cancer Research, Vol. 75, No. 20, 15.10.2015, p. 4272-4282.

Research output: Contribution to journalArticle

Heilmann, S, Ratnakumar, K, Langdon, EM, Kansler, ER, Kim, IS, Campbell, NR, Perry, EB, McMahon, AJ, Kaufman, CK, Van Rooijen, E, Lee, W, Iacobuzio-Donahue, CA, Hynes, RO, Zon, LI, Xavier, JB & White, RM 2015, 'A quantitative system for studying metastasis using transparent zebrafish', Cancer Research, vol. 75, no. 20, pp. 4272-4282. https://doi.org/10.1158/0008-5472.CAN-14-3319
Heilmann S, Ratnakumar K, Langdon EM, Kansler ER, Kim IS, Campbell NR et al. A quantitative system for studying metastasis using transparent zebrafish. Cancer Research. 2015 Oct 15;75(20):4272-4282. https://doi.org/10.1158/0008-5472.CAN-14-3319
Heilmann, Silja ; Ratnakumar, Kajan ; Langdon, Erin M. ; Kansler, Emily R. ; Kim, Isabella S. ; Campbell, Nathaniel R. ; Perry, Elizabeth B. ; McMahon, Amy J. ; Kaufman, Charles K. ; Van Rooijen, Ellen ; Lee, William ; Iacobuzio-Donahue, Christine A. ; Hynes, Richard O. ; Zon, Leonard I. ; Xavier, Joao B. ; White, Richard M. / A quantitative system for studying metastasis using transparent zebrafish. In: Cancer Research. 2015 ; Vol. 75, No. 20. pp. 4272-4282.
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