Abstract
Proteomics is a rapidly developing field and it opens new horizons in many research areas of life sciences. In the field of medicine, proteomics promises to accelerate the discovery ofnew drug targets and protein disease markers useful for in vitro diagnosis. In this article, we review the current proteomics technologies for biomarker discovery and validation, which include two-dimensional gel electrophoresis, one- and two-dimensional liquid chromatography, and proteomic microarrays. We will also review proteomic strategies for protein-protein interactions and identification of posttranslational modifications. Selection of the more effective technology or combination of technologies is required to maximize the interpretation and utility of the data.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 9-17 |
| Number of pages | 9 |
| Journal | Proceedings of the American Thoracic Society |
| Volume | 4 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 2007 |
| Externally published | Yes |
Keywords
- 2D electrophoresis
- Multiple dimensional chromatography
- Post-translational modification
- Proteomic microarrays
- Proteomics
ASJC Scopus subject areas
- Pulmonary and Respiratory Medicine