A proteomic analysis of human hemodialysis fluid

Henrik Molina, Jakob Bunkenborg, G. Hanumanthu Reddy, Babylakshmi Muthusamy, Paul J. Scheel, Akhilesh Pandey

Research output: Contribution to journalArticle

Abstract

The vascular compartment is an easily accessible compartment that provides an opportunity to measure analytes for diagnostic, prognostic, or therapeutic indications. Both serum and plasma have been analyzed extensively by proteomic approaches in an effort to catalog all proteins and polypeptides. Limitations of such approaches in obtaining a comprehensive catalog of proteins include the fact that a handful of proteins constitute over 90% of plasma protein content and that the renal glomeruli filter out proteins and polypeptides that are smaller than 66 kDa from blood. We chose to study hemodialysis fluid because it contains a higher concentration of small proteins and polypeptides and is also simultaneously depleted of the most abundant proteins present in blood. Using gel electrophoresis in combination with LC-MS/MS, we identified 292 proteins of which greater than 70% had not been previously identified from serum or plasma. More than half of the proteins identified from the hemodialysis fluid were smaller than 40 kDa. We also found 50 N-terminally acetylated peptides that allowed us to unambiguously map the N termini of mature forms of the corresponding proteins. Several identified proteins, including cytokines, were only present as predicted transcripts in data bases and thus represent novel proteins. The proteins identified in this study could serve as biomarkers in serum using more sensitive methods such as ELISA-specific antibodies.

Original languageEnglish (US)
Pages (from-to)637-650
Number of pages14
JournalMolecular and Cellular Proteomics
Volume4
Issue number5
DOIs
StatePublished - May 2005

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Proteomics
Renal Dialysis
Fluids
Proteins
Peptides
Blood
Serum
Plasmas
Biomarkers
Electrophoresis
Blood Vessels
Blood Proteins
Gels
Enzyme-Linked Immunosorbent Assay
Databases
Cytokines
Kidney
Antibodies

ASJC Scopus subject areas

  • Biochemistry

Cite this

Molina, H., Bunkenborg, J., Reddy, G. H., Muthusamy, B., Scheel, P. J., & Pandey, A. (2005). A proteomic analysis of human hemodialysis fluid. Molecular and Cellular Proteomics, 4(5), 637-650. https://doi.org/10.1074/mcp.M500042-MCP200

A proteomic analysis of human hemodialysis fluid. / Molina, Henrik; Bunkenborg, Jakob; Reddy, G. Hanumanthu; Muthusamy, Babylakshmi; Scheel, Paul J.; Pandey, Akhilesh.

In: Molecular and Cellular Proteomics, Vol. 4, No. 5, 05.2005, p. 637-650.

Research output: Contribution to journalArticle

Molina, H, Bunkenborg, J, Reddy, GH, Muthusamy, B, Scheel, PJ & Pandey, A 2005, 'A proteomic analysis of human hemodialysis fluid', Molecular and Cellular Proteomics, vol. 4, no. 5, pp. 637-650. https://doi.org/10.1074/mcp.M500042-MCP200
Molina H, Bunkenborg J, Reddy GH, Muthusamy B, Scheel PJ, Pandey A. A proteomic analysis of human hemodialysis fluid. Molecular and Cellular Proteomics. 2005 May;4(5):637-650. https://doi.org/10.1074/mcp.M500042-MCP200
Molina, Henrik ; Bunkenborg, Jakob ; Reddy, G. Hanumanthu ; Muthusamy, Babylakshmi ; Scheel, Paul J. ; Pandey, Akhilesh. / A proteomic analysis of human hemodialysis fluid. In: Molecular and Cellular Proteomics. 2005 ; Vol. 4, No. 5. pp. 637-650.
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